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International Journal of Legal Medicine

, Volume 133, Issue 2, pp 347–351 | Cite as

Mitochondrial DNA control region diversity in a population from Parana state—increasing the Brazilian forensic database

  • M. M. Poletto
  • M. Malaghini
  • J. S. Silva
  • M.G. Bicalho
  • K. Braun-PradoEmail author
Original Article
  • 85 Downloads

Abstract

The entire mitochondrial DNA (mtDNA) control region (nucleotide position 16024-576) sequences were obtained through Sanger sequencing method for 122 individuals from Parana state, South of Brazil. We observed a total of 108 different haplotypes of which 97 were unique and 11 were shared by more than one individual. The haplogroups were classified according to the updated mtDNA phylogeny, by EMMA (estimating mitochondrial haplogroups using a maximum likelihood approach). Our results revealed the predominance of Amerindian haplogroups with a frequency of 49.2% of the population sample, followed by European lineages with 38.5% and 12.3% of African lineages. Parana population sample set presented a high haplotype diversity (0.9976) and the random match probability was 0.0106. The phylogenetical findings and the diversity indices confirm the high genetic heterogeneity of this population and suggest a high informativeness of mtDNA analyses in forensic cases. The population data will contribute to increase the Brazilian mtDNA database for forensic purposes and it is available through EMPOP (European DNA Profiling Group mitochondrial DNA population database) under the accession number EMP00714.

Keywords

Mitochondrial DNA Control region Genetic population data Forensic application Parana Brazil 

Notes

Acknowledgments

The authors would like to acknowledge the Federal University of Parana, Laboratory of Immunogenetics and Histocompatibility and Scientific Policy of Parana.

Funding information

This work was supported by the Foundation of Federal University of Parana (FUNPAR).

Compliance with ethical standards

This study was performed according to Brazilian Federal laws and was approved by the Human Research Ethics Committee of the Federal University of Parana.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

414_2018_1886_MOESM1_ESM.doc (122 kb)
ESM 1 (DOC 121 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratório de Imunogenética e HistocompatibilidadeUniversidade Federal do ParanáCuritibaBrazil
  2. 2.Laboratório de Genética Molecular ForensePolícia Científica do ParanáCuritibaBrazil
  3. 3.Laboratório de Genética Molecular HumanaUniversidade Federal do ParanáCuritibaBrazil

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