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International Journal of Legal Medicine

, Volume 132, Issue 1, pp 219–227 | Cite as

Characterization of bone diagenesis by histology in forensic contexts: a human taphonomic study

  • Yann DelannoyEmail author
  • Thomas Colard
  • Catherine Cannet
  • Vadim Mesli
  • Valéry Hédouin
  • Guillaume Penel
  • Bertrand Ludes
Original Article

Abstract

The diagenesis of a bone in the postmortem period causes an identifiable deterioration in histology. This degradation is characterized by a collagenous alteration, which can be observed very early. In order to develop a method for determining a postmortem interval for medico-legal use, two ribs collected from six human bodies were studied prospectively over 2 years. Each bone was studied after staining with Sirius red to demonstrate the degradation of collagen as a function of time. This study demonstrated a time-based bone alteration characterized by the architectural degradation of the lamellar bone, without any microbial influence in this postmortem period. The staining was carried out by using Sirius red and correlated this alteration with a collagenic degradation by chemical hydrolysis owing to the affinity of this dye to the amino acids lysine, hydroxylysine, and arginine. Our work asserts that human bone samples that were studied in a controlled environment and analyzed for 24 months underwent a diagenetic trajectory whose main element was collagen hydrolysis.

Keywords

Forensic medicine Anthropolgy Bone diagenesis Collagen hydrolysis 

Notes

Acknowledgements

We would like to express our sincere appreciation to American Journal Experts, Guillaume Falgayrac and Claire Louria, for their technical and scientific assistance with the preparation of this manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Yann Delannoy
    • 1
    • 2
    • 3
    • 4
    • 5
    Email author
  • Thomas Colard
    • 2
    • 3
    • 4
  • Catherine Cannet
    • 6
  • Vadim Mesli
    • 2
    • 3
  • Valéry Hédouin
    • 2
    • 3
  • Guillaume Penel
    • 2
    • 4
  • Bertrand Ludes
    • 1
    • 7
  1. 1.Institut Médico-Légal de ParisUniversité Paris Descartes Sorbonne CitéParisFrance
  2. 2.CHU de LILLELilleFrance
  3. 3.EA 7367 Forensic Taphonomy Unit - Lille Forensic InstituteLille UniversityLilleFrance
  4. 4.Littoral Côte d’Opale, EA 4490, PMOI, Physiopathologie des Maladies Osseuses inflammatoiresLille UniversityLilleFrance
  5. 5.Institut de Médecine Légale – CHRU de LilleLille CedexFrance
  6. 6.Laboratoire d’Histomorphométrie de Médecine Légale, Faculté de MédecineStrasbourgFrance
  7. 7.CNRS UMR 5288, AMISToulouse UniversityToulouseFrance

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