Evaluation of InnoTyper® 21 in a sample of Rio de Janeiro population as an alternative forensic panel
The use of bi-allelic markers such as retrotransposable element insertion polymorphisms or Innuls (for insertion/null) can overcome some limitations of short tandem repeat (STR) loci in typing forensic biological evidence. This study investigated the efficiency of the InnoTyper® 21 Innul markers in an urban admixed population sample in Rio de Janeiro (n = 40) and one highly compromised sample collected as evidence by the Rio de Janeiro police. No significant departures from Hardy-Weinberg equilibrium were detected after the Bonferroni correction (α′ ≈ 0.05/20, p < 0.0025), and no significant linkage disequilibrium was observed between markers. Assuming loci independence, the cumulative random match probability (RMP) was 2.3 × 10−8. A lower mean Fis value was obtained for this sample population compared with those of three North American populations (African-American, Southwest Hispanic, US Caucasian). Principal component analysis with the three North American populations and one from 21 East Asian population showed that African Americans segregated as an independent group while US Caucasian, Southwest Hispanic, East Asian, and Rio de Janeiro populations are in a single large heterogeneous group. Also, a full Innuls profile was produced from an evidence sample, despite the DNA being highly degraded. In conclusion, this system is a useful complement to standard STR kits.
KeywordsForensic genetics Transposable element markers Innuls Rio de Janeiro Brazil
- 4.Gill P, Werrett DJ, Budowle B, Guerrieri R (2004) An assessment of whether SNPs will replace STRs in national DNA databases—joint considerations of the DNA working group of the European Network of Forensic Science Institutes (ENFSI) and the Scientific Working Group on DNA Analysis Methods (SWGDAM). Sci Justice 44:51–53. doi:10.1016/S1355-0306(04)71685-8 CrossRefPubMedGoogle Scholar
- 6.Sudhir K, Carter A (2010) Method for genetic detection using interspersed genetic elements. US patent # 7,794,983Google Scholar
- 13.Brown H, Thompson R, Murphy G, Peters D, LaRue B, Kiing J, Montgomery AH, Carroll M, Baus J, Sinha S, Wendt FR, Song B, Chakraborty R, Budowle B, Sinha SK (2017) Development and validation of a novel multiplexed DNA analysis system, InnoTyper®21. Forens Sci Int Genet 29:80–99. doi:10.1016/j.fsigen.2017.03.017 CrossRefGoogle Scholar
- 16.Oostdik K, Lenz K, Nye J, Schelling K, Yet D, Bruski S, Strong J, Buchanan C, Sutton J, Linner J, Frazier N, Young H, Matthies L, Sage A, Hahn J, Wells R, Williams N, Price M, Koehler J, Staples M, Swango KL, Hill C, Oyerly K, Duke W, Katzilierakis L, Ensenberger MG, Bourdeau JM, Sprecher CJ, Krenke B, Storts DR (2014) Developmental validation of the PowerPlex® fusion system for analysis of casework and reference samples: a 24-locus multiplex for new database standards. Forensic Sci Int Genet 12:69–76. doi:10.1016/j.fsigen.2014.04.013 CrossRefPubMedGoogle Scholar