International Journal of Legal Medicine

, Volume 131, Issue 3, pp 605–620 | Cite as

Developmental validation of the HomyGene19+14Y System

  • Weian Du
  • Ling Chen
  • Hong Liu
  • Pingming Qiu
  • Fayuan Li
  • Jing Gao
  • Yu Zhou
  • Bangchao Wang
  • Chao Liu
Original Article
First Online:
Received:
Accepted:

Abstract

The HomyGene19+14Y System (HG19+14Y) is a PCR-based amplification kit that enables typing of 18 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, Penta E, TPOX, TH01, vWA), 14 widely used Y chromosome STR (Y-STR) loci (Y_GATA_H4, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYS456, DYS458, DYS635), and amelogenin. This multiplex system was designed for the simultaneous analysis of amelogenin-Y allele mutation, single-source searches, kinship (including familial searching), mixture profiles, international data sharing, and other forensic applications. In this study, the multiplex system was validated for sensitivity, specificity, DNA mixtures, stability, precision, stutter, reproducibility, parallel tests, PCR-based conditions, and population analysis according to the Scientific Working Group on DNA Analysis Methods (SWGDAM) developmental validation guidelines. A total of 212 alleles were detected for the 18 autosomal STR loci among 528 Guangdong Han individuals, and 431 haplotypes were found for 14 Y-STRs among 452 unrelated males. The combined match probability (CMP) of the HG19+14Y System was calculated as 2.39 × 10−29. All the validation results showed that the HG19+14Y System would be a robust, reliable, highly polymorphic, and informative forensic kit.

Keywords

STR Short tandem repeat Multiplex PCR Validation DNA typing Forensic science 
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Notes

Acknowledgements

This work was supported by a grant from Science and Technology Planning Project of Guangdong Province, China (Grant No. 2013B021500010), Natural Science Foundation of Guangdong Province (Grant No. 2014A030310025), and Medical Science and Technology Research Foundation of Guangdong Province (A2015043).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

414_2016_1505_MOESM1_ESM.docx (35 kb)
Table S1 The allele frequencies and forensic parameters of 18 autosomal STR loci in Guangdong Han population (n = 528) (DOCX 35 kb)
414_2016_1505_MOESM2_ESM.docx (24 kb)
Table S2 The allele frequencies and GD values of 14 Y-STR loci in Guangdong Han male population (n = 452) (DOCX 24 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Weian Du
    • 1
  • Ling Chen
    • 1
  • Hong Liu
    • 2
  • Pingming Qiu
    • 1
  • Fayuan Li
    • 3
  • Jing Gao
    • 4
  • Yu Zhou
    • 4
  • Bangchao Wang
    • 4
  • Chao Liu
    • 1
    • 2
  1. 1.School of Forensic MedicineSouthern Medical UniversityGuangzhouChina
  2. 2.Guangzhou Forensic Science InstituteGuangdong Province Key Laboratory of Forensic GeneticsGuangzhouChina
  3. 3.AGCU ScienTech IncorporationWuxiChina
  4. 4.HOMY GeneTech IncorporationFoshanChina

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