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International Journal of Legal Medicine

, Volume 131, Issue 2, pp 293–301 | Cite as

Compatibility of DNA IQ™, QIAamp® DNA Investigator, and QIAsymphony® DNA Investigator® with various fingerprint treatments

  • Sze-wah Lin
  • Stephen C. Y. IpEmail author
  • Tze-tsun Lam
  • Tung-fai Tan
  • Wai-lung Yeung
  • Wai-ming TamEmail author
Original Article

Abstract

Latent fingerprint and touch DNA are the two most important contact evidence for individualization in forensic science which provide complementary information that can lead to direct and unequivocal identification of the culprit. In order to retrieve useful information from both fingerprints and DNA, which are usually mingled together, one strategy is to perform fingerprint examination prior to DNA analysis since common DNA sampling technique such as swabbing could disturb or even destroy fingerprint details. Here, we describe the compatibility of three automatic DNA extraction systems, namely, DNA IQ™, QIAamp® DNA Investigator, and QIAsymphony® DNA Investigator®, with respective to the effects of various fingerprint detection techniques. Our results demonstrate that Super Glue fingerprint treatment followed by DNA IQ™ extraction shows better effectiveness in DNA profiling. Aluminum powder dusting offers the least interference to the three DNA extraction systems above. Magnetic powder dusting, on the other hand, strongly impedes DNA recovery. Physical Developer is the most intrusive, which yields profiles with poor quality, including lower peak heights, poor peak height ratios, and poor intra-color balance. In terms of the choice of extraction method, DNA IQ™ system is recommended for sampling after fingerprint treatments, but not the two DNA Investigator systems.

Keywords

Forensic DNA typing Fingerprint Super Glue Magnetic powder Aluminum powder Physical Developer 

Notes

Acknowledgments

We thank Dr. W.M. Sin, the Government Chemist of the Government Laboratory, Dr. F.C. Kwok, the Assistant Government Chemist, Mr. B.K.K. Cheung, the Chief Chemist, Mr. S.C. Dove, the Senior Superintendent of Police, and Mr. C.T. Leung, the Superintendent of Police, for their support and approval of the publication of this work. We thank Dr. K. M. Lai and Mr. C. F. Wu for helpful comments on the manuscript and Ms. S. F. Lam, Mr. Y.M. Lee, and Mr. M.H. Lo for their technical support for this work. We also thank other members of our laboratory for invaluable discussions.

Supplementary material

414_2016_1447_MOESM1_ESM.pdf (150 kb)
ESM 1 Supplementary Fig. 1 The yield of DNA obtained from (a) undiluted, and (b) 100-folded diluted buffy coat samples (mean ± S.E.M., n =10) by IQ, QC and SYM after various non-/semi-porous fingerprint treatments. Dotted lines indicate the optimum amount of input DNA for amplification. Different letters (a, b, c, d, e, f & g) indicate statistical significance (P < 0.05). IQ, QC and SYM denote the DNA IQTM, QIAcube, and QIAsymphony® systems respectively. Alu, Mag, Glu, and Dye(s) denote aluminum powder, magnetic powder, Super Glue, and fluorescent dye(s), respectively. Supplementary Fig. 2 The yield of DNA obtained from (a) undiluted, and (b) 100-folded diluted buffy coat samples (mean ± S.E.M., n =10) by IQ, QC and SYM after various porous fingerprint treatments. Dotted lines indicate the optimum amount of input DNA for amplification. Different letters (a, b & c) indicate statistical significance (P < 0.05). IQ, QC and SYM denote the DNA IQTM, QIAcube, and QIAsymphony® systems respectively. Ind, NFN, and PD denote indanedione-zinc, ninhydrin, and Physical Developer, respectively. (PDF 1207 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Forensic Science Division, Government Laboratory, Homantin Government OfficesHong KongChina
  2. 2.Identification Bureau, Hong Kong Police Force, Police HeadquartersHong KongChina

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