International Journal of Legal Medicine

, Volume 131, Issue 2, pp 351–358 | Cite as

Case report: on the use of the HID-Ion AmpliSeq™ Ancestry Panel in a real forensic case

  • C. HollardEmail author
  • C. Keyser
  • T. Delabarde
  • A. Gonzalez
  • C. Vilela Lamego
  • V. Zvénigorosky
  • B. Ludes
Case Report


In the absence of any other conclusive forensic evidence, DNA profiling is the method of choice for body identification. This study focuses on the case of a carbonized corpse whose complete autosomal short tandem repeat (STR) profile could not lead to direct identification by the investigators. To assist in the progress of investigation, we endeavoured to determine the biogeographical origin and eye colour of the deceased individual. Along with Y chromosome and mitochondrial DNA analyses, we applied a next-generation sequencing (NGS) approach to the study of ancestry informative markers (AIMs) using the HID-Ion AmpliSeq™ Ancestry Panel launched by Thermo Fisher Scientific. This work gave us the opportunity to test this new technology in a real forensic case. Although this study highlights the benefits of such a combined approach, as it markedly improves the specificity of the biogeographical profile, it also underlines the need for the accurate characterization of a larger collection of reference populations and the necessity of caution in data interpretation.


Next-generation sequencing Ancestry Degraded DNA Unidentified body 

Supplementary material

414_2016_1425_MOESM1_ESM.pdf (659 kb)
ESM 1 (PDF 658 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • C. Hollard
    • 1
    Email author
  • C. Keyser
    • 1
    • 2
    • 3
  • T. Delabarde
    • 1
    • 3
  • A. Gonzalez
    • 1
  • C. Vilela Lamego
    • 2
    • 3
  • V. Zvénigorosky
    • 2
  • B. Ludes
    • 2
    • 3
    • 4
    • 5
  1. 1.Institute of Legal MedicineUniversité de StrasbourgStrasbourgFrance
  2. 2.AMIS Laboratory, CNRS UMR 5288Université de ToulouseToulouseFrance
  3. 3.Institut National de la Transfusion Sanguine (INTS)ParisFrance
  4. 4.Institute of Legal MedicineParisFrance
  5. 5.Université Paris DescartesParisFrance

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