Population genetic analysis of the GlobalFiler STR loci in 748 individuals from the Kazakh population of Xinjiang in northwest China
- 369 Downloads
The six-dye GlobalFiler™ Express PCR amplification kit incorporates 21 commonly used autosomal short tandem repeat (STR) loci and three gender determination loci. In this study, we analyzed the GlobalFiler STR loci on 748 unrelated individuals from a Chinese Kazakh population of Xinjiang, China. No significant deviations from Hardy-Weinberg equilibrium and linkage disequilibrium were observed within and between 21 autosomal STR loci. SE33 showed the greatest power of discrimination in Kazakh population. The combined power of discrimination of Kazakh was 99.999999999999999999999996797 %. No significant differences of allele frequencies were observed between Kazakh and Uyghur at all 15 tested STR loci, as well as Mongolian. Significant differences were only observed between Kazakh and the other Chinese populations at TH01. Multiple STR loci showed significant differences between Kazakh and Arab, as well as South Portuguese. The multidimensional scaling plot (MDS) plot and neighbor-joining tree also showed Kazakh is genetically close to Uyghur.
KeywordsShort tandem repeat GlobalFiler Kazakh Xinjiang
This study was supported by grants from the National Science Foundation of China (31271338), Project of Chinese Ministry of Education (113022A) and the National High Technology Research and Development Program (2012AA021802).
Compliance with ethical standard
Conflict of interest
The authors declare that they have no competing interests.
- 3.Wang DY, Gopinath S, Lagacé RE, Norona W, Hennessy LK, Short ML, Mulero JJ (2015) Developmental validation of the GlobalFiler® Express PCR Amplification Kit: a 6-dye multiplex assay for the direct amplification of reference samples. Forensic Sci Int-Genet 19:148–155. doi: 10.1016/j.fsigen.2015.07.013 CrossRefPubMedGoogle Scholar
- 4.Flores S, Sun J, King J, Budowle B (2014) Internal validation of the GlobalFiler (TM) Express PCR Amplification Kit for the direct amplification of reference DNA samples on a high-throughput automated workflow. Forensic Sci Int-Genet 10:33–39. doi: 10.1016/j.fsigen.2014.01.005 CrossRefPubMedGoogle Scholar
- 8.Yuan JY, Wang XY, Shen CM, Liu WJ, Yan JW, Wang HD, Pu HW, Wang YL, Yang G, Zhang YD, Meng HT, Jing H, Zhu BF (2014) Genetic profile characterization and population study of 21 autosomal STR in Chinese Kazak ethnic minority group. Electrophoresis 35(4):503–510. doi: 10.1002/elps.201300398 CrossRefPubMedGoogle Scholar
- 12.Zhao F, Wu X, Cai G, Xu C (2003) The application of Modified-Powerstates software in forensic biostatistics (in Chinese). Chinese Journal of Forensic Medicine 18(5):297–298Google Scholar
- 16.Li L, Xu J, Liu X, Chen W, Xia M, Yang S, Jiang P, Ma T, Yang Y, Qian J, Sun H, Hu R, Miqin, Feng Z, Zuo Y, Zhou R, Ping Y, Zhou H, Zhao Z, Jin L, Li S (2015) Population data of 15 short tandem repeat loci in 1084 individuals from six Han and four ethnic populations in China. Forensic Sci Int-Genet 19:146–147. doi: 10.1016/j.fsigen.2015.06.015