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International Journal of Legal Medicine

, Volume 129, Issue 2, pp 245–249 | Cite as

Evaluation of a 49 InDel Marker HID panel in two specific populations of South America and one population of Northern Africa

  • R. S. Moura-Neto
  • R. Silva
  • I. C. Mello
  • T. Nogueira
  • A. A. Al-Deib
  • B. LaRue
  • J. King
  • B. Budowle
Short Communication

Abstract

The majority of STR loci are not ideal for the analysis of forensic samples with degraded and/or low template DNA. One alternative to overcome these limitations is the use of bi-allelic markers, which have low mutation rates and shorter amplicons. Human identification (HID) InDel marker panels have been described in several countries, including Brazil. The commercial kit available is, however, mostly suitable for Europeans, with lower discrimination power for other population groups. Recently, a combination of 49 InDel markers used in four different ethnic groups in the USA has been shown to be more informative than another panel from Portugal, already tested in a Rio de Janeiro sample. However, these 49 InDels have yet to be applied to other admixed or isolated populations. We assessed the efficiency of this panel in two urban admixed populations (Rio de Janeiro, Brazil; Tripoli, Libya) and one isolated Native Brazilian community. All markers are in Hardy-Weinberg equilibrium (HWE) after the Bonferroni correction, and no Linkage disequilibrium was detected. Assuming loci independence and no substructure effect, cumulative RMP was 2.7×10−18, 1.5×10−20, and 4.5×10−20 for Native Brazilian, Rio de Janeiro, and Tripoli populations, respectively. The overall Fst value was 0.05512. Rio de Janeiro and Tripoli showed similar admixture levels, however for Native Brazilians one parental cluster represented over 60 % of the total parental population. We conclude that this panel is suitable for HID on these urban populations, but is less efficient for the isolated group.

Keywords

Forensic genetics Tripoli Libya Rio de Janeiro Brazil Amerindian 

Notes

Acknowledgments

This work was supported in part by research grants from FAPERJ, CNPq, CAPES, and INMETRO.

Supplementary material

414_2014_1137_MOESM1_ESM.docx (128 kb)
ESM 1 (DOCX 128 kb)
414_2014_1137_MOESM2_ESM.docx (236 kb)
ESM 2 (DOCX 236 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • R. S. Moura-Neto
    • 1
    • 2
  • R. Silva
    • 3
  • I. C. Mello
    • 3
  • T. Nogueira
    • 4
  • A. A. Al-Deib
    • 5
  • B. LaRue
    • 6
  • J. King
    • 6
  • B. Budowle
    • 6
    • 7
  1. 1.Laboratório de Biologia Molecular Forense, Instituto de BiologiaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Instituto Nacional de MetrologiaQualidade e TecnologiaDuque de CaxiasBrazil
  3. 3.Instituto de Biofisica Carlos Chagas FilhoUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  4. 4.Instituto de Biologia do ExercitoRio de JaneiroBrazil
  5. 5.Faculty of the Medical TechnologyUniversity of TripoliTripoliLibya
  6. 6.Institute of Applied Genetics, Department of Forensic and Investigative GeneticsUniversity of North Texas Health Science CenterForth WorthUSA
  7. 7.Center of Excellence in Genomic Medicine Research (CEGMR)King Abdulaziz UniversityJeddahSaudi Arabia

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