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International Journal of Legal Medicine

, Volume 127, Issue 1, pp 63–64 | Cite as

Population data of 12 X-STR loci in a North of Portugal sample

  • Laura Cainé
  • Sérgio Costa
  • Maria F. Pinheiro
Population Data

DNA markers on the X-chromosome (ChrX) have been shown to be powerful tools for solving complex relationship cases [1].

The ChrX contains four linkage groups located at Xp22.2, Xq12, Xq26, and Xq28 that can provide independent genotype information. At present, it is proposed that it is preferable to use clusters to define haplotypes in forensic practice [2].

The X-Decaplex (DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08, and DXS7423) has been used by the GHEP-ISFG to make several studies [3, 4] and to solve forensic cases.

The Investigator Argus X-12 kit (Qiagen) includes 12 X-STRs located in four different linkage groups (linkage group 1: DXS10148, DXS10135, DXS8378; linkage group 2: DXS7132, DXS10079, DXS10074; linkage group 3: DXS10103, HPRTB, DXS10101; linkage group 4: DXS10146, DXS10134, DXS7423).

The Investigator ArgusX-12 Kit is a multiplex application kit that amplifies 12X-chromosomal STR loci highly suited for paternity testing [5],...

Keywords

Linkage Group Polymorphism Information Content Unique Haplotype Paternity Testing Haplotype Data 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Supplementary material

414_2012_672_MOESM1_ESM.xlsx (12 kb)
Table S 1 Forensic parameters for 12 X-STR loci in the tested population of the North of Portugal (150 males, 73 females). (XLSX 12 kb)
414_2012_672_MOESM2_ESM.xlsx (12 kb)
Table S 2 Allele frequencies of the 12 X-STR loci in the tested population of the North of Portugal (150 males, 73 females). (XLSX 11 kb)
414_2012_672_MOESM3_ESM.xlsx (10 kb)
Table S 3 Distribution of STR haplotypes for the Chr X linkage groups (n = 150 males). (XLSX 9 kb)
414_2012_672_MOESM4_ESM.xlsx (11 kb)
Table S 4a Haplotype frequencies on Chr X linkage group 1 (n = 150 males). (XLSX 11 kb)
414_2012_672_MOESM5_ESM.xlsx (11 kb)
Table S 4b Haplotype frequencies on Chr X linkage group 2 (n = 150 males). (XLSX 10 kb)
414_2012_672_MOESM6_ESM.xlsx (11 kb)
Table S 4c Haplotype frequencies on Chr X linkage group 3 (n = 150 males). (XLSX 10 kb)
414_2012_672_MOESM7_ESM.xlsx (11 kb)
Table S 4d Haplotype frequencies on Chr X linkage group 4 (n = 150 males). (XLSX 11 kb)

References

  1. 1.
    Becker D, Rodig H, Augustin C, Edelmann J, Götz F, Hering S, Szibor R, Brabetz W (2008) Population genetic evaluation of eight X-chromosomal short tandem repeat loci using Mentype Argus X-8 PCR amplification kit. Forensic Sci Int Genet 2:69–74PubMedCrossRefGoogle Scholar
  2. 2.
    Szibor R (2007) The X chromosome in forensic science: past, present and future. Molecular forensics. Wiley. 103–126Google Scholar
  3. 3.
    Gusmão L, Sánchez-Diz P, Alves C et al (2008) A GEP-ISFG collaborative study on the optimization of an X-STR decaplex: data on 15 Iberian and Latin American populations. Int J Legal Med. doi: 10.1007/s00414-008-0309-4
  4. 4.
    Zarrabeitia MT, Pinheiro F, de Pancorbo MM et al (2009) Analysis of 10 -linked tetranucleotide markers in mixed and isolated populations. Forensic Sci Int Genet 3:63–66PubMedCrossRefGoogle Scholar
  5. 5.
    Cainé L, Carvalho R, Costa S, Pereira M, Pinheiro MF Interest of X chromosome (Argus X-12 kit) in complex kinship analysis. Forensic Sci Int Genet Suppl Ser. doi: 10.1016/j.fsigss.2011.08.103
  6. 6.
    Edelmann J, Lutz-Bonengel S, Naue J, Hering S (2012) X-chromosomal haplotype frequencies of four linkage groups using the Investigator Argus X-12 Kit. Forensic Sci Int Genet 6:24–34Google Scholar
  7. 7.
    Tomas C, Pereira V, Morling N (2012) Analysis of 12 X-STRs in Greenlanders, Danes and Somalis using Argus X-12. Int J Legal Med 126 (1):121–128Google Scholar
  8. 8.
    Walsh PS, Metzer DA, Higuchi R (1991) CHELEX® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Laura Cainé
    • 1
  • Sérgio Costa
    • 1
  • Maria F. Pinheiro
    • 1
  1. 1.PortoPortugal

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