International Journal of Legal Medicine

, Volume 124, Issue 2, pp 165–170 | Cite as

MtDNA control region sequence polymorphisms and phylogenetic analysis of Malay population living in or around Kuala Lumpur in Malaysia

  • S. Maruyama
  • C. Nohira-Koike
  • K. Minaguchi
  • P. Nambiar
Short Communication

Abstract

Control region polymorphisms in the mitochondrial DNA of 124 unrelated individuals from the Malay population living in or around Kuala Lumpur in Malaysia were investigated and phylogenetic haplogroup lineages were determined. The intergenic COII/tRNALys 9-bp deletion, 3010 and 5178 mutations, and several coding region polymorphisms were examined to discriminate some phylogenetic haplogroups. Sequence comparison of the control regions led to the identification of 117 mitochondrial haplotypes, in which 103 types were observed in only one individual and the other nine types were shared by more than two individuals. Gene diversity was estimated to be 0.997. Phylogenetic haplogroup determination revealed that the gene pool of the modern Malay population in Malaysia consisted mainly of southeast Asian, east Asian, unidentified and unique, and aboriginal southeast-specific haplogroups. These results suggest a multi-original nature for the modern Malay population. The present database may help not only in personal identification but also in determining geographic origin in forensic casework in Malaysian, Southeast Asian and East Asian populations.

Keywords

Mitochondrial DNA Polymorphism Control region Haplogroup Malay population 

Supplementary material

414_2009_355_MOESM1_ESM.xls (202 kb)
ESM Table S1Variable nucleotide positions in 124 Malay samples compared to reference sequences. (XLS 201 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • S. Maruyama
    • 1
  • C. Nohira-Koike
    • 1
  • K. Minaguchi
    • 1
  • P. Nambiar
    • 2
  1. 1.Department of Forensic OdontologyTokyo Dental CollegeChiba CityJapan
  2. 2.Department of General Dental Practice and Oral & Maxillofacial Imaging, Faculty of DentistryUniversity of MalayaKuala LumpurMalaysia

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