International Journal of Legal Medicine

, Volume 124, Issue 2, pp 91–98

Homogeneity in mitochondrial DNA control region sequences in Swedish subpopulations

  • Andreas O. Tillmar
  • Michael D. Coble
  • Thomas Wallerström
  • Gunilla Holmlund
Original Article

Abstract

In order to promote mitochondrial DNA (mtDNA) testing in Sweden we have typed 296 Swedish males, which will serve as a Swedish mtDNA frequency database. The tested males were taken from seven geographically different regions representing the contemporary Swedish population. The complete mtDNA control region was typed and the Swedish population was shown to have high haplotype diversity with a random match probability of 0.5%. Almost 47% of the tested samples belonged to haplogroup H and further haplogroup comparison with worldwide populations clustered the Swedish mtDNA data together with other European populations. AMOVA analysis of the seven Swedish subregions displayed no significant maternal substructure in Sweden (FST = 0.002). Our conclusion from this study is that the typed Swedish individuals serve as good representatives for a Swedish forensic mtDNA database. Some caution should, however, be taken for individuals from the northernmost part of Sweden (provinces of Norrbotten and Lapland) due to specific demographic conditions. Furthermore, our analysis of a small sample set of a Swedish Saami population confirmed earlier findings that the Swedish Saami population is an outlier among European populations.

Keywords

Mitochondrial DNA Sweden Saami Control region Demography 

Supplementary material

414_2009_354_MOESM1_ESM.doc (374 kb)
ESM1 (DOC 374 kb)

References

  1. 1.
    Budowle B, Allard MW, Wilson MR, Chakraborty R (2003) Forensics and mitochondrial DNA: applications, debates, and foundations. Annu Rev Genomics Hum Genet 4:119–141CrossRefPubMedGoogle Scholar
  2. 2.
    Gill P, Ivanov PL, Kimpton R et al (1994) Identification of the remains of the Romanov family by DNA analysis. Nat Genet 6:130–135CrossRefPubMedGoogle Scholar
  3. 3.
    Buckleton J, Walsh SJ, Harbison S (2005) Nonautosomal forensic markers. In: Buckleton J, Triggs CM, Walsh SJ (eds) Forensic DNA evidence interpretation. CRC, Boca RatonGoogle Scholar
  4. 4.
    Parson W, Bandelt HJ (2007) Extended guidelines for mtDNA typing of population data in forensic science. Forensic Sci Int Genet 1:13–19CrossRefPubMedGoogle Scholar
  5. 5.
    Parson W, Dür A (2007) EMPOP—a forensic mtDNA database. Forensic Sci Int Genet 1:88–92CrossRefPubMedGoogle Scholar
  6. 6.
    Tambets K, Rootsi S, Kivisild T et al (2004) The western and eastern roots of the Saami—the story of genetic “outliers” told by mitochondrial DNA and Y chromosomes. Am J Hum Genet 74:661–682CrossRefPubMedGoogle Scholar
  7. 7.
    Svonni M (2005) The Sami language—a historical mystery. In: Sami Parliament & Ministry of Agriculture, Food and Consumer Affairs (ed), The Sami—an indigenous people in Sweden, Kiruna, pp 50–51Google Scholar
  8. 8.
    Lundmark L (2008) Stulet land. Svensk makt på samisk mark. Ordfront, StockholmGoogle Scholar
  9. 9.
    Hansen LI (1996) Interaction between Northern European subarctic societies during the Middle Ages: indigenous peoples, peasants and state builders. In: Rindal M (ed) Two studies on the Middle Ages, KULTs skriftserie 66. Research Council of Oslo, Oslo, pp 31–95Google Scholar
  10. 10.
    Hansen LI, Olsen B (2004) Samenes historie fram til 1750. Cappelen Akademisk Forlag, OsloGoogle Scholar
  11. 11.
    Blankholm HP (2008) Southern Scandinavia. In: Bailey G, Spikins P (eds) Mesolithic Europe. Cambridge University Press, New York, pp 107–131Google Scholar
  12. 12.
    Bjerk HB (2008) Norwegian Mesolithic trends: a review. In: Bailey G, Spikins P (eds) Mesolithic Europe. Cambridge University Press, New York, pp 60–106Google Scholar
  13. 13.
    Hörnberg G, Bohlin E, Hellberg E et al (2005) Effects of Mesolithic hunter-gatherers on local vegetation in a non-uniform glacio-isostatic land uplift area, northern Sweden. Veg Hist Archaeobot 15:13–26CrossRefGoogle Scholar
  14. 14.
    Lindén M (2006) Glaciodynamics, deglacial landforms and isostatic uplift during the last deglaciation of Norrbotten, LUNDAQUA Theses 54. Lund University, SwedenGoogle Scholar
  15. 15.
    Bergman I, Zachrisson O (2007) Early Mesolithic hunter-gatherers and landscape acquisition by the Arctic Circle. J North Stud 1–2:123–142Google Scholar
  16. 16.
    Östlund O (2005) Stenåldersboplatsen i Kangos—nästan 10 000 år i glömska. Den tidigaste kolonisationen i Norrbotten. Norrbotten, pp. 141–149Google Scholar
  17. 17.
    Zvelebil M (2008) Innovating hunter-gatherers: the Mesolithic in the Baltic. In: Bailey G, Spikins P (eds) Mesolithic Europe. Cambridge University Press, New York, pp 18–59Google Scholar
  18. 18.
    Svanberg I, Tydén M (2005) Tusen år av invandring: en svensk kulturhistoria. Dialogos, StockholmGoogle Scholar
  19. 19.
    Myrdal J (2003) Digerdöden, pestvågor och ödeläggelse. Ett perspektiv på senmedeltidens Sverige. Sällskapet Runica et Mediævalia. Scripta minora 9. StockholmGoogle Scholar
  20. 20.
    Karlsson AO, Wallerström T, Götherström A, Holmlund G (2006) Y-chromosome diversity in Sweden—a long-time perspective. Eur J Hum Genet 14:963–970CrossRefPubMedGoogle Scholar
  21. 21.
    Lindblom B, Holmlund G (1988) Rapid DNA purification for restriction fragment length polymorphism analysis. Gene Anal Tech 5:97–101CrossRefPubMedGoogle Scholar
  22. 22.
    Brandstätter A, Peterson CT, Irwin JA et al (2004) Mitochondrial DNA control region sequences from Nairobi (Kenya): inferring phylogenetic parameters for the establishment of a forensic database. Int J Leg Med 118(5):294–306CrossRefGoogle Scholar
  23. 23.
    Irwin J, Saunier J, Strouss K et al (2007) Development and expansion of high quality control region databases to improve forensic mtDNA evidence interpretation. Forensic Sci Int Genet 1(2):154–157CrossRefPubMedGoogle Scholar
  24. 24.
    Anderson S, Bankier AT, Barrell BG et al (1981) Sequence and organization of the human mitochondrial genome. Nature 290:457–465CrossRefPubMedGoogle Scholar
  25. 25.
    Andrews RM, Kubacka I, Chinnery PF et al (1999) Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA. Nat Genet 23:147CrossRefPubMedGoogle Scholar
  26. 26.
    Brandstätter A, Niederstätter H, Pavlic M et al (2006) Generating population data for the EMPOP database—an overview of the mtDNA sequencing and data evaluation processes considering 273 Austrian control region sequences as example. Forensic Sci Int 166(2, 3):164–175PubMedGoogle Scholar
  27. 27.
    Abu-Amero KK, Gonzalez AM, Larruga JM et al (2007) Eurasian and African mitochondrial DNA influences in the Saudi Arabian population. BMC Evol Biol 7:32CrossRefPubMedGoogle Scholar
  28. 28.
    Alshamali F, Brandstätter A, Zimmermann B, Parson W (2008) Mitochondrial DNA control region variation in Dubai, United Arab Emirates. Forensic Sci Int Genet 2:e9–e10CrossRefPubMedGoogle Scholar
  29. 29.
    Chen F, Wang SY, Zhang RZ et al (2008) Analysis of mitochondrial DNA polymorphisms in Guangdong Han Chinese. Forensic Sci Int Genet 2:150–153CrossRefPubMedGoogle Scholar
  30. 30.
    Helgason A, Hickey E, Goodacre S et al (2001) MtDNA and the islands of the North Atlantic: estimating the proportions of Norse and Gaelic ancestry. Am J Hum Genet 68:723–737CrossRefPubMedGoogle Scholar
  31. 31.
    Hedman M, Brandstätter A, Pimenoff V et al (2007) Finnish mitochondrial DNA HVS-I and HVS-II population data. Forensic Sci Int 172:171–178CrossRefPubMedGoogle Scholar
  32. 32.
    Irwin J, Saunier J, Strouss K et al (2008) Mitochondrial control region sequences from northern Greece and Greek Cypriots. Int J Legal Med 122:87–89CrossRefPubMedGoogle Scholar
  33. 33.
    Lehocký I, Baldovič M, Kádaši L, Metspalu E (2008) A database of mitochondrial DNA hypervariable regions I and II sequences of individuals from Slovakia. Forensic Sci Int Genet 2(4):53–59CrossRefGoogle Scholar
  34. 34.
    Saunier JL, Irwin J, Just RS et al (2008) Mitochondrial control region sequences from a U.S. “Hispanic” population sample. Forensic Sci Int Genet 2:e19–e23CrossRefPubMedGoogle Scholar
  35. 35.
    Turchi C, Buscemi L, Previdere C et al (2008) Italian mitochondrial DNA database: results of a collaborative exercise and proficiency testing. Int J Leg Med 122:199–204CrossRefGoogle Scholar
  36. 36.
    Excoffier LLG, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50PubMedGoogle Scholar
  37. 37.
    Tetzlaff S, Brandstätter A, Wegener R et al (2007) Mitochondrial DNA population data of HVS-I and HVS-II sequences from a northeast German sample. Forensic Sci Int 172:218–224CrossRefPubMedGoogle Scholar
  38. 38.
    Passarino G, Cavalleri GL, Lin AA et al (2002) Different genetic components in the Norwegian population revealed by the analysis of mtDNA and Y chromosome polymorphisms. Eur J Hum Genet 10:521–529CrossRefPubMedGoogle Scholar
  39. 39.
    Pakendorf B, Stoneking M (2005) Mitochondrial DNA and human evolution. Annu Rev Genomics Hum Genet 6:165–183CrossRefPubMedGoogle Scholar
  40. 40.
    Selinge KG (1996) Östergötland, Förhistoria I: Nationalencyklopedin, Höganäs, pp 480–481Google Scholar
  41. 41.
    Hörfors O (2000) Östergötland, Förhistoria I: Nationalencyklopedin Supplement III Malmö, pp 596Google Scholar
  42. 42.
    Selinge KG (1995) Småland, Förhistoria, I: Nationalencyklopedin, Höganäs, pp 7–8Google Scholar
  43. 43.
    Andersson Palm L (2000) Folkmängden i Sveriges socknar och kommuner 1571–1997. Med särskild hänsyn till perioden 1571–1751. Historiska institutionen, University of GuthenburgGoogle Scholar
  44. 44.
    Baudou E (1995) Norrlands forntid—ett historiskt perspektiv. Cewe-förlaget, BjästaGoogle Scholar
  45. 45.
    Wallerström T (1995) Norrbotten, Sverige och medeltiden. Problem kring makt och bosättning i en europeisk periferi I. Lund Studies in Medieval Archaeology, vol 15. Almqvist & Wiksell, Stockholm, p 1Google Scholar
  46. 46.
    Broadbent N (2004) The search for a past: the prehistory of the indigenous Saami in northern coastal Sweden. In: Herva VP (ed) People, material culture and environment in the north. Proceedings of the 22nd Nordic Archaeological Conference University of Oulu, pp 13–25Google Scholar
  47. 47.
    Manker E (1953) The nomadism of the Swedish mountain lapps: the Siidas and their migratory routes in 1945. Acta Lapponica 7. Nordiska museet, StockholmGoogle Scholar
  48. 48.
    Hultblad F (1968) Övergång från nomadism till agrar bosättning i Jokkmokk socken. Acta Lapponica XIV. Nordiska museet, StockholmGoogle Scholar
  49. 49.
    Wallerström T (2000) The Saami between east and west in the Middle Ages. An archaeological contribution to the history of reindeer breeding. Acta Borealia 17:3–39CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Andreas O. Tillmar
    • 1
  • Michael D. Coble
    • 2
  • Thomas Wallerström
    • 3
  • Gunilla Holmlund
    • 1
  1. 1.Department of Forensic Genetics and Forensic ToxicologyNational Board of Forensic MedicineLinköpingSweden
  2. 2.Armed Forces DNA Identification LaboratoryRockvilleUSA
  3. 3.Department of Archaeology and Religious StudiesNorwegian University of Science and Technology (NTNU)TrondheimNorway

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