International Journal of Legal Medicine

, Volume 123, Issue 1, pp 75–79

Non-invasive prenatal paternity testing from maternal blood

  • Jasenka Wagner
  • Snježana Džijan
  • Damir Marjanović
  • Gordan Lauc
Technical Note

DOI: 10.1007/s00414-008-0292-9

Cite this article as:
Wagner, J., Džijan, S., Marjanović, D. et al. Int J Legal Med (2009) 123: 75. doi:10.1007/s00414-008-0292-9


Prenatal paternity analysis can be performed only after invasive sampling of chorionic villi or amnionic fluid. Aiming to enable noninvasive paternity testing, we attempted to amplify fetal alleles from maternal plasma. Cell-free DNA was isolated from plasma of 20 pregnant women and amplified with ampFLSTR Identifiler and ampFLSTR Yfiler kits. Unfortunately, autosomal fetal alleles were heavily suppressed by maternal DNA, and the only locus that was reliably amplified with AmpFLSTR Identifiler kit was amelogenin, which revealed only fetal gender. Much better success was obtained with AmpFLSTR Yfiler kit, which, in the case of male fetuses, successfully amplified between six and 16 fetal loci. All amplified fetal alleles matched the alleles of their putative fathers, confirming the tested paternity. To the best of our knowledge, this is a first report of noninvasive prenatal paternity testing.


Prenatal paternity testing Cell-free fetal DNA STR loci 

Supplementary material

414_2008_292_MOESM1_ESM.pdf (729 kb)
ESM 1(PDF 746 kb)
414_2008_292_MOESM2_ESM.pdf (789 kb)
ESM 2(PDF 807 kb)
414_2008_292_MOESM3_ESM.pdf (739 kb)
ESM 3(PDF 756 kb)
414_2008_292_MOESM4_ESM.pdf (748 kb)
ESM 4(PDF 766 kb)
414_2008_292_MOESM5_ESM.pdf (816 kb)
ESM 5(PDF 835 kb)
414_2008_292_MOESM6_ESM.pdf (695 kb)
ESM 6(PDF 711 kb)
414_2008_292_MOESM7_ESM.pdf (685 kb)
ESM 7(PDF 701 kb)
414_2008_292_MOESM8_ESM.pdf (693 kb)
ESM 8(PDF 709 kb)
414_2008_292_MOESM9_ESM.pdf (561 kb)
ESM 9(PDF 574 kb)
414_2008_292_MOESM10_ESM.pdf (679 kb)
ESM 10(PDF 695 kb)
414_2008_292_MOESM11_ESM.pdf (549 kb)
ESM 11(PDF 562 kb)
414_2008_292_MOESM12_ESM.pdf (555 kb)
ESM 12(PDF 568 kb)
414_2008_292_MOESM13_ESM.pdf (543 kb)
ESM 13(PDF 555 kb)
414_2008_292_MOESM14_ESM.pdf (693 kb)
ESM 14(PDF 709 kb)
414_2008_292_MOESM15_ESM.pdf (788 kb)
ESM 15(PDF 806 kb)
414_2008_292_MOESM16_ESM.pdf (730 kb)
ESM 16(PDF 747 kb)
414_2008_292_MOESM17_ESM.pdf (725 kb)
ESM 17(PDF 742 kb)
414_2008_292_MOESM18_ESM.pdf (816 kb)
ESM 18(PDF 835 kb)
414_2008_292_MOESM19_ESM.pdf (685 kb)
ESM 19(PDF 701 kb)
414_2008_292_MOESM20_ESM.pdf (688 kb)
ESM 20(PDF 704 kb)
414_2008_292_MOESM21_ESM.pdf (561 kb)
ESM 21(PDF 574 kb)
414_2008_292_MOESM22_ESM.pdf (610 kb)
ESM 22(PDF 624 kb)
414_2008_292_MOESM23_ESM.pdf (566 kb)
ESM 23(PDF 579 kb)
414_2008_292_MOESM24_ESM.pdf (574 kb)
ESM 24(PDF 588 kb)
414_2008_292_MOESM25_ESM.pdf (632 kb)
ESM 25(PDF 647 kb)
414_2008_292_MOESM26_ESM.pdf (543 kb)
ESM 26(PDF 555 kb)
414_2008_292_MOESM27_ESM.pdf (540 kb)
ESM 27(PDF 553 kb)
414_2008_292_MOESM28_ESM.xls (28 kb)
Supplementary Table(XLS 28 kb)
414_2008_292_MOESM29_ESM.doc (50 kb)
Supplementary electropherograms(DOC 51 kb)

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jasenka Wagner
    • 1
  • Snježana Džijan
    • 1
  • Damir Marjanović
    • 2
  • Gordan Lauc
    • 1
    • 2
  1. 1.University of Osijek School of Medicine, DNA LaboratoryOsijekCroatia
  2. 2.Genos d.o.o.OsijekCroatia

Personalised recommendations