International Journal of Legal Medicine

, Volume 121, Issue 2, pp 158–162 | Cite as

Changes in colour of different human tissues as a marker of age

  • Alexander PilinEmail author
  • František Pudil
  • Vladimír Bencko
Technical Note


This study deals with age estimation based on colour changes of human tissue from the intervertebral discs, Achilles tendon and rib cartilage. The investigated colour changes are the result of the accumulation of non-enzymatic browning products in the tissue. Samples of excised tissues were photographed with a digital camera and the pictures were evaluated using the image analysis processor Lucia G 4.11 processor. The values of the intensities of the RGB channels (MeanRed, Mean Green, MeanBlue) and parameters from the IHS system (MeanSaturation, HueTypical, HueVariation, BrightVariation and MeanBrightness) were evaluated. The results confirm that colour changes of some tissues depend on ageing and are a good tool for age estimation.


Age estimation Colour changes of tissues Non-enzymatic browning Image analysis AGEs Lucia G 



This work was supported by a research grant NK7281-4 from the Internal Grant Agency of the Ministry of Health of the Czech Republic.


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Alexander Pilin
    • 1
  • František Pudil
    • 2
  • Vladimír Bencko
    • 3
  1. 1.Institute of Forensic Medicine and Toxicology of the First Faculty of MedicineCharles University and General Teaching Hospital in PraguePraha 2Czech Republic
  2. 2.Institute of Chemistry and Analysis of FoodInstitute of Chemical Technology in PraguePraha 6Czech Republic
  3. 3.Institute of Hygiene and Epidemiology of the First Faculty of MedicineCharles University in PraguePraha 2Czech Republic

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