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International Journal of Legal Medicine

, Volume 120, Issue 3, pp 160–164 | Cite as

Short amplicon STR multiplex for stain typing

  • P. WiegandEmail author
  • R. Klein
  • G. Braunschweiger
  • C. Hohoff
  • B. Brinkmann
Technical Note

Abstract

We developed a short tandem repeat (STR) typing kit based on DNA database systems that are included in, for example, the Interpol Standard Set of Loci recommendations (i.e., TH01, VWA, D3S1358, FGA) and the gender typing system Amelogenin. Two different multiplex sets were tested using the fluorescent dyes FAM, JOE, and VIC. The PCR results were compared to the commercially available AmpFISTR Blue kit, which contains the STRs D3S1358, VWA, and FGA. The advantage of our multiplex compared with the Blue kit was the generation of shorter amplicons (<200 bp) and the higher combined power of discrimination.

Keywords

DNA database Mini STRs PCR multiplex Stain typing 

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • P. Wiegand
    • 1
    Email author
  • R. Klein
    • 1
  • G. Braunschweiger
    • 2
  • C. Hohoff
    • 3
  • B. Brinkmann
    • 3
  1. 1.Department of Legal MedicineUniversity HospitalUlmGermany
  2. 2.genFOR GmbHGrafschaft-GelsdorfGermany
  3. 3.Institute of Legal MedicineMünsterGermany

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