International Journal of Legal Medicine

, Volume 120, Issue 3, pp 160–164 | Cite as

Short amplicon STR multiplex for stain typing

  • P. WiegandEmail author
  • R. Klein
  • G. Braunschweiger
  • C. Hohoff
  • B. Brinkmann
Technical Note


We developed a short tandem repeat (STR) typing kit based on DNA database systems that are included in, for example, the Interpol Standard Set of Loci recommendations (i.e., TH01, VWA, D3S1358, FGA) and the gender typing system Amelogenin. Two different multiplex sets were tested using the fluorescent dyes FAM, JOE, and VIC. The PCR results were compared to the commercially available AmpFISTR Blue kit, which contains the STRs D3S1358, VWA, and FGA. The advantage of our multiplex compared with the Blue kit was the generation of shorter amplicons (<200 bp) and the higher combined power of discrimination.


DNA database Mini STRs PCR multiplex Stain typing 


  1. 1.
    Schneider PM, Martin PD (2001) Criminal DNA databases: the European situation. Forensic Sci Int 119:232–238CrossRefPubMedGoogle Scholar
  2. 2.
    Jobling MA, Gill P (2004) Encoded evidence: DNA in forensic analysis. Nat Rev Genet 10:739–751CrossRefGoogle Scholar
  3. 3.
    Gill P, Whitaker J, Flaxman C, Brown N, Buckleton J (2001) An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA. Forensic Sci Int 112:17–40CrossRefGoogle Scholar
  4. 4.
    Wiegand P, Kleiber M (2001) Less is more—length reduction of STR amplicons using redesigned primers. Int J Legal Med 114:285–287PubMedCrossRefGoogle Scholar
  5. 5.
    Hellmann A, Rohleder U, Schmitter H, Wittig M (2001) STR typing of human telogen hairs—a new approach. Int J Legal Med 114:269–273PubMedCrossRefGoogle Scholar
  6. 6.
    Ross J, Parson W, Furac I, Kubat M, Holland M (2001) Multiplex PCR amplification of eight STR loci in Austrian and Croatian Caucasian populations. Int J Legal Med 115:57–60CrossRefPubMedGoogle Scholar
  7. 7.
    Butler JM, Shen Y, McCord BR (2003) The development of reduced size STR amplicons as tools for analysis of degraded DNA. J Forensic Sci 48:1054–1064PubMedGoogle Scholar
  8. 8.
    Wallin JM, Buoncristiani MR, Lazaruk KD, Fildes N, Holt CL, Walsh S (1998) TWGDAM validation of the AmpFlSTR Blue PCR amplification kit for forensic casework analysis. J Forensic Sci 43:854–870PubMedGoogle Scholar
  9. 9.
    Coticone SR, Oldroyd N, Philips H, Foxall P (2004) Development of the AmpFlSTR SEfiler PCR amplification kit: a new multiplex containing the highly discriminating ACTBP2 (SE33) locus. Int J Legal Med 118:224–234CrossRefPubMedGoogle Scholar
  10. 10.
    Schlenk J, Seidl S, Braunschweiger G, Betz P, Lederer T (2004) Development of a 13-locus PCR multiplex system for paternity testing. Int J Legal Med 118:55–61CrossRefPubMedGoogle Scholar
  11. 11.
    Junge A, Lederer T, Braunschweiger G, Madea B (2003) Validation of the multiplex kit genRESMPX-2 for forensic casework analysis. Int J Legal Med 117:317–325CrossRefPubMedGoogle Scholar
  12. 12.
    Brinkmann B, Junge A, Meyer E, Wiegand P (1998) Population genetic diversity in relation to microsatellite heterogeneity. Hum Mutat 11:135–144CrossRefPubMedGoogle Scholar
  13. 13.
    Neuhuber F, Radacher M, Meisner N, Tutsch-Bauer E (1999) Nine STR markers plus amelogenin (AMFlSTR profiler plus): a forensic study in an Austrian population. Int J Legal Med 113:60–62CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • P. Wiegand
    • 1
    Email author
  • R. Klein
    • 1
  • G. Braunschweiger
    • 2
  • C. Hohoff
    • 3
  • B. Brinkmann
    • 3
  1. 1.Department of Legal MedicineUniversity HospitalUlmGermany
  2. 2.genFOR GmbHGrafschaft-GelsdorfGermany
  3. 3.Institute of Legal MedicineMünsterGermany

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