International Journal of Legal Medicine

, Volume 120, Issue 1, pp 42–48

Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit

  • Ulrike Schmidt
  • Sabine Lutz-Bonengel
  • Hans-Joachim Weisser
  • Timo Sänger
  • Stefan Pollak
  • Ulrike Schön
  • Thomas Zacher
  • Wolfgang Mann
Technical Note

Abstract

In forensic DNA analysis, improvement of DNA typing technologies has always been an issue. It has been shown that DNA amplification in low volumes is a suitable way to enhance the sensitivity and efficiency of amplification. In this study, DNA amplification was performed on a flat, chemically structured glass slide in 1-μl reaction volumes from cell line DNA contents between 1,000 and 4 pg. On-chip DNA amplification reproducibly yielded full allelic profiles from as little as 32 pg of template DNA. Applicability on the simultaneous amplification of 15 short tandem repeats and of a segment of the Amelogenin gene, which are routinely used in forensic DNA analysis, is shown. The results are compared to conventional in-tube amplification carried out in 25-μl reaction volumes.

Keywords

STR On-chip amplification DNA typing Low-volume amplification Fragment length analysis 

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Ulrike Schmidt
    • 1
  • Sabine Lutz-Bonengel
    • 1
  • Hans-Joachim Weisser
    • 1
  • Timo Sänger
    • 1
  • Stefan Pollak
    • 1
  • Ulrike Schön
    • 2
  • Thomas Zacher
    • 2
  • Wolfgang Mann
    • 2
  1. 1.Institute of Legal MedicineAlbert Ludwig University FreiburgFreiburgGermany
  2. 2.Alopex GmbHKulmbachGermany

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