International Journal of Legal Medicine

, Volume 118, Issue 4, pp 245–247 | Cite as

Genetic identification of forensically important flesh flies (Diptera: Sarcophagidae)

  • Richard Zehner
  • Jens Amendt
  • Svenja Schütt
  • Jan Sauer
  • Roman Krettek
  • Dalibor Povolný
Short Communication
First Online:
Received:
Accepted:

Abstract

Unequivocal identification of fly specimens is an essential requirement in forensic entomology. However, not all species can be determined at every developmental stage, which is illustrated by the flesh flies (Diptera: Sarcophagidae), important members of the necrophagous insect fauna. Up to now no suitable key for the identification of the immature stages of this family of flies exists. DNA analysis of selected mitochondrial genes was applied to solve this problem. Sequence data of selected regions of the CO I and ND 5 genes of the most important European flesh fly taxa associated with cadavers are presented, which can act as reference standards for species determination.

Keywords

Sarcophagidae Forensic entomology DNA analysis Species determination 
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References

  1. Amendt J, Zehner R, Krettek R (2004) Forensic Entomology. Naturwissenschaften 91:51–65CrossRefPubMedGoogle Scholar
  2. Anderson GS, Cervenka VJ (2002) Insects associated with the body: their use and analyses. In: Haglund WD, Sorg MH (eds) Advances in forensic taphonomy—method, theory and archaeological perspectives. CRC Press, Boca Raton, pp 173–200Google Scholar
  3. Byrd JH, Castner JL (2001) Insects of forensic importance. In: Byrd JH, Castner JL (eds) Forensic entomology—the utility of arthropods in legal investigations. CRC Press, Boca Raton, pp 287–302Google Scholar
  4. Caterino MS, Cho S, Sperling FA (2000) The current state of insect molecular systematics: a thriving Tower of Babel. Annu Rev Entomol 45:1-54PubMedGoogle Scholar
  5. Higley LG, Haskell NH (2001) Insect development and forensic entomology. In: Byrd JH, Castner JL (eds) Forensic entomology—the utility of arthropods in legal investigations. CRC Press, Boca Raton pp 287–302Google Scholar
  6. Kalendar R (1999) Institute of Biotechnology, University of Helsinki, Finland, Oligos© version 8.31. http://www.biocenter.helsinki.fi/bi/bare-1_html/oligos.htm
  7. Kocher TD, Thomas WK, Meyer A, Edwards SV, Pääbo S, Villablance FX, Wilson AC (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc Natl Acad Sci U S A 86:6196–6200PubMedGoogle Scholar
  8. Loxdale HD, Lushai G (1998) Molecular markers in entomology. Bull Entomol Res 88: 577–600Google Scholar
  9. Malgorn Y, Coquoz R (1999) DNA typing for identification of some species of Calliphoridae. An interest in forensic entomology. Forensic Sci Int 102:111–119CrossRefPubMedGoogle Scholar
  10. Parson W, Pegoraro K, Niederstatter H, Foger M, Steinlechner M (2000) Species identification by means of the cytochrome b gene. Int J Legal Med114: 23–28CrossRefGoogle Scholar
  11. Sambrook J, Russel DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
  12. Schröder H, Klotzbach H, Elias S, Augustin C, Pueschel K (2003) Use of PCR-RFLP for differentiation of calliphorid larvae (Diptera, Calliphoridae) on human corpses. Forensic Sci Int 132:76–81CrossRefPubMedGoogle Scholar
  13. Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87:651–701Google Scholar
  14. Sperling FA, Anderson GS, Hickey DA (1994) A DNA-based approach to the insect species used for postmortem interval estimation. J Forensic Sci 39:418–427PubMedGoogle Scholar
  15. Stevens J, Wall R (2001) Genetic relationships between blowflies (Calliphoridae) of forensic importance. Forensic Sci Int 120:116–123CrossRefPubMedGoogle Scholar
  16. Swofford DL (1991) PAUP: phylogenetic analysis using Parsimony version 3.1 computer program. Illinois Natural History Survey, Champaign, IllinoisGoogle Scholar
  17. Wallman JF, Donnellan SC (2001) The utility of mitochondrial DNA sequences for the identification of forensically important blowflies (Diptera: Calliphoridae) in southeastern Australia. Forensic Sci Int 120:60–67CrossRefPubMedGoogle Scholar
  18. Wells JD, Sperling FA (2001) DNA-based identification of forensically important Chrysomyinae (Diptera: Calliphoridae). Forensic Sci Int 120:110–115CrossRefPubMedGoogle Scholar
  19. Wells JD, Pape T, Sperling FA (2001) DNA-based identification and molecular systematics of forensically important Sarcophagidae (Diptera). J Forensic Sci 46:1098–102PubMedGoogle Scholar
  20. Zehner R, Zimmermann S, Mebs D, Bratzke H (1998) RFLP and sequence analysis of the cytochrome b gene of selected animals and man: methology and forensic application. Int J Legal Med 111:323–327CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Richard Zehner
    • 1
  • Jens Amendt
    • 1
  • Svenja Schütt
    • 1
  • Jan Sauer
    • 1
  • Roman Krettek
    • 1
  • Dalibor Povolný
    • 2
  1. 1.Center of Legal MedicineUniversity of Frankfurt Frankfurt/MainGermany
  2. 2.Department of ZoologyMendel UniversityBrnoCzech Republic

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