International Journal of Legal Medicine

, Volume 119, Issue 1, pp 1–9 | Cite as

Separate analysis of DYS385a and b versus conventional DYS385 typing: is there forensic relevance?

  • Harald Niederstätter
  • Burkhard Berger
  • Herbert Oberacher
  • Anita Brandstätter
  • Christian G. Huber
  • Walther Parson
Original Article

Abstract

In order to determine to what extent the separate analysis of both copies of DYS385 improves Y-chromosomal short tandem repeat (Y-STR) haplotyping, we followed a recently published protocol for the separate amplification of DYS385a and DYS385b with modifications and compared the results with those obtained by conventional analysis in a population sample comprising 133 unrelated Caucasian males from Austria. Additionally, we typed all markers of the minimal haplotype (minHT) and a set of Y-chromosomal single nucleotide polymorphisms (Y-SNPs) in order to interpret the STR data depending on the Y-SNP haplogroup structure. The separate amplification of DYS385a and b improved the power of discrimination of this marker when compared to the results obtained with the conventional non-locus-discriminating amplification strategy. However, the degree of this improvement varied greatly between different haplogroups and was found to be highest in clade K. In the forensically relevant context of the minHT, the separate analysis of the DYS385 alleles had no effect on the differentiation of paternal lineages in our study. Furthermore, the amplicon lengths of 700–780 base pairs obtained in the course of the locus-discriminating approach restrict the applicability of this amplification strategy to high quality DNA samples.

Keywords

Y chromosome DYS385 Y-STR Y-SNP haplogroups Population study 

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Harald Niederstätter
    • 1
  • Burkhard Berger
    • 1
  • Herbert Oberacher
    • 1
  • Anita Brandstätter
    • 1
  • Christian G. Huber
    • 2
  • Walther Parson
    • 1
  1. 1.Institute of Legal MedicineMedical University of InnsbruckInnsbruckAustria
  2. 2.Instrumental Analysis and BioanalysisSaarland University Building 9.2SaarbrückenGermany

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