Chromosoma

, Volume 110, Issue 2, pp 65–74 | Cite as

Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells

  • Richard R. Adams
  • Mark D. Eckley
  • Paola Vagnarelli
  • Sally P. Wheatley
  • Dietlind L. Gerloff
  • Alastair M. Mackay
  • Phyllis A. Svingen
  • Scott H. Kaufmann
  • William C. Earnshaw
Original Article

Abstract.

The inner centromere protein (INCENP), which has previously been described in chicken, frog and mouse, is required for correct chromosome segregation and cytokinesis. We have identified the human INCENP gene by library screening and reverse transcription-polymerase chain reaction (RT-PCR) and localized it to chromosomal region 11q12. HsINCENP is a single-copy gene that consists of 17 exons and covers 25 kb of genomic DNA. The gene is expressed at highest levels in the colon, testis and prostate, consistent with its likely role in cell proliferation. HsINCENP encodes a highly basic protein of 915 amino acids that localizes to metaphase chromosomes and to the mitotic spindle and equatorial cortex at anaphase. Recently we showed that INCENP is stockpiled in a complex with the Aurora-B/XAIRK2 kinase in Xenopus eggs. Here we demonstrate that, consistent with such an interaction, the two proteins colocalize on human metaphase chromosomes. Levels of Aurora-B are increased in several human cancers, and we show here that HsINCENP protein levels are also significantly increased in several colorectal cancer cell lines.

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Richard R. Adams
    • 1
  • Mark D. Eckley
    • 2
  • Paola Vagnarelli
    • 1
  • Sally P. Wheatley
    • 1
  • Dietlind L. Gerloff
    • 1
  • Alastair M. Mackay
    • 2
  • Phyllis A. Svingen
    • 3
  • Scott H. Kaufmann
    • 3
  • William C. Earnshaw
    • 1
  1. 1.Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, Kings Buildings, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, UK
  2. 2.Department of Cell Biology and Anatomy, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
  3. 3.Division of Oncology Research, Mayo Clinic, 200 First St., S.W., Rochester, MN 55905, USA

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