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Chromosoma

, Volume 108, Issue 6, pp 384–392 | Cite as

Homolog pairing and meiotic progression in Coprinus cinereus

  • Libo Li
  • Erin E. Gerecke
  • Miriam E. Zolan

Abstract.

We have used fluorescence in situ hybridization to examine homolog pairing during the synchronous meiosis of the basidiomycete Coprinus cinereus. Using spread preparations of meiotic nuclei, we confirmed previous studies that showed that at 6 h post-karyogamy essentially all meiotic nuclei are in pachytene. We found that homolog pairing occurs rapidly after karyogamy, that a 1 Mb chromosome does not associate more quickly than a 2.5 Mb chromosome, and that interstitial, single-copy sites can associate stably prior to nucleolar fusion. Analysis of two probes for the same pair of homologs revealed that by 4 h after karyogamy each chromosome examined was at least partially paired in all meiotic cells. In addition, these studies showed that chromatin condensation increases after pairing and that chromatin shows stable compaction at pachytene.

Keywords

Chromatin Condensation Meiotic Cell Homolog Pairing Meiotic Progression Meiotic Nucleus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Libo Li
    • 1
  • Erin E. Gerecke
    • 1
  • Miriam E. Zolan
    • 1
  1. 1.Department of Biology, Indiana University, 1001 East 3rd Street, Bloomington, IN 47405, USAIN

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