Chromosoma

, Volume 108, Issue 3, pp 200–207 | Cite as

New ribosomal RNA gene locations in Gossypium hirsutum mapped by meiotic FISH

  • Yuanfu Ji
  • Marcos De Donato
  • Charles F. Crane
  • Wayne A. Raska
  • M. Nurul Islam-Faridi
  • Thomas D. McKnight
  • H. James Price
  • David M. Stelly

Abstract.

In this study we have mapped newly identified rDNA loci in Gossypium hirsutum. Four new minor 18S-26S rDNA loci, in addition to the sites previously identified, were mapped using fluorescence in situ hybridization (FISH) to heterozygous translocation (NT) quadrivalents (IVs). The newly detected 18S-26S rDNA loci were mapped to the right arms of chromosomes 8, 9, 15, 17, 19, 20, and 23 and the left arms of chromosomes 5, 11, 12, and 14. Using the rDNA loci as common reference points, we detected several erroneous arm assignments in the previously published map of NT breakpoints. The data are summarized in the form of an integrated map for all 17 known rDNA loci, relative to centromeres, telomeres, and NT breakpoints. This information will facilitate future locus-specific research on rRNA gene evolution and function.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Yuanfu Ji
    • 1
  • Marcos De Donato
    • 1
  • Charles F. Crane
    • 1
  • Wayne A. Raska
    • 1
  • M. Nurul Islam-Faridi
    • 1
  • Thomas D. McKnight
    • 2
  • H. James Price
    • 1
  • David M. Stelly
    • 1
  1. 1.Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USAUS
  2. 2.Department of Biology, Texas A&M University, College Station, TX 77843, USAUS

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