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Chromosoma

, Volume 108, Issue 1, pp 10–25 | Cite as

CHD1 interacts with SSRP1 and depends on both its chromodomain and its ATPase/helicase-like domain for proper association with chromatin

  • Dawn E. Kelley
  • David G. Stokes
  • Robert P. Perry
Original articles

Abstract.

CHD1, an Mr∼200,000 protein that contains a chromodomain (C), an ATPase/helicase-like domain (H) and a DNA-binding domain (D), was previously shown to be associated with decompacted interphase chromatin in mammalian cells and with transcriptionally active puffs and interbands in Drosophila polytene chromosomes. We now show by transient transfection experiments with genes expressing wild-type and mutant forms of CHD1 that both the C and H domains are essential for its proper association with chromatin. We also present evidence for an in vivo interaction between CHD1 and a novel HMG box-containing protein, SSRP1, which involves an amino-terminal segment of CHD1 that does not include the chromodomain. Immunocytochemical analyses indicated that CHD1 and SSRP1 colocalize in both mammalian nuclei and Drosophila polytene chromosomes.

Keywords

Mammalian Cell Transient Transfection Proper Association Mutant Form Transfection Experiment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Dawn E. Kelley
    • 1
  • David G. Stokes
    • 1
  • Robert P. Perry
    • 1
  1. 1.Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USAUS

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