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Chromosoma

, Volume 107, Issue 2, pp 80–86 | Cite as

Characterisation of the nucleolar organising regions during the cell cycle in two varieties of Petunia hybrida as visualised by fluorescence in situ hybridisation and silver staining

  • M. B. Montijn
  • R. ten Hoopen
  • P. F. Fransz
  • J. L. Oud
  • N. Nanninga
ORIGINAL ARTICLE

Abstract. 

The cell cycle-dependent spatial position, morphology and activity of the four nucleolar organising regions (NORs) of the Petunia hybrida cultivar Mitchell and the inbred line V26 have been analysed. Application of the silver staining technique and fluorescence in situ hybridisation on fixed root-tip material revealed that these interspecific hybrids possess four NORs of which only those of chromosome 2 are active during interphase, which implies that the NOR activity is not of parental origin. However, at the end of mitosis, activity of all NOR regions could be detected, suggesting that the high demand for ribosomes at this stage of the cell cycle requires temporal activity of all NORs. Using actin DNA probes as markers in fluorescence in situ hybridisation experiments enabled the identification of the individual petunia chromosomes.

Keywords

Cell Cycle Inbred Line High Demand Spatial Position Temporal Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • M. B. Montijn
    • 1
  • R. ten Hoopen
    • 1
  • P. F. Fransz
    • 1
  • J. L. Oud
    • 1
  • N. Nanninga
    • 1
  1. 1.BioCentrum Amsterdam, Institute for Molecular Cell Biology, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The NetherlandsNL

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