Chromosoma

, Volume 109, Issue 7, pp 482–489 | Cite as

Evolutionary conservation of kinetochore protein sequences in plants

  • Rogier ten Hoopen
  • Renate Manteuffel
  • Jaroslav Doležel
  • Ludmilla Malysheva
  • Ingo Schubert
Original Article

Abstract.

The evolutionary conservation of structural/functional kinetochore proteins has been studied on isolated nuclei and pro-/metaphase chromosomes of mono- and dicot plants. The cross-reactivities of antibodies against human CENPC, CENPE and CENPF, and against maize CENPCa with the centromeric regions of mitotic chromosomes of Vicia faba and/or Hordeum vulgare are shown. Putative homologs of the kinetochore protein SKP1 (suppressor of kinetochore protein 1p of yeast) were found in both species and of CBF5p (centromere binding factor 5 of yeast) in barley. Antibodies against synthetic peptides derived from partial sequences encoding these proteins were produced and recognized the centromeric regions on mitotic chromosomes as detected by indirect immunofluorescence.

Keywords

Peptide Protein Sequence Binding Factor Synthetic Peptide Partial Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • Rogier ten Hoopen
    • 1
  • Renate Manteuffel
    • 1
  • Jaroslav Doležel
    • 2
  • Ludmilla Malysheva
    • 1
  • Ingo Schubert
    • 1
  1. 1.Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany
  2. 2.Institute of Experimental Botany, 77200 Olomouc, Czech Republic

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