Chromosome painting in meiosis reveals pairing of specific chromosomes in polyploid Solanum species
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Analysis of chromosome pairing has been an important tool to assess the genetic similarity of homologous and homoeologous chromosomes in polyploids. However, it is technically challenging to monitor the pairing of specific chromosomes in polyploid species, especially for plant species with a large number of small chromosomes. We developed oligonucleotide-based painting probes for four different potato chromosomes. We demonstrate that these probes are robust enough to monitor a single chromosome throughout the prophase I of meiosis in polyploid Solanum species. Cultivated potato (Solanum tuberosum, 2n = 4x = 48) is an autotetraploid. We demonstrate that the four copies of each potato chromosome pair as a quadrivalent in 66–78% of the meiotic cells at the pachytene stage. Solanum demissum (2n = 6x = 72) is a hexaploid and has been controversial regarding its nature as an autopolyploid or allopolyploid. Interestingly, no hexavalent pairing was observed in meiosis. Instead, we observed three independent bivalents in 83–98% of the meiotic cells at late diakinesis and early metaphase I for the four chromosomes. These results suggest that S. demissum has evolved into a cytologically stable state with predominantly bivalent pairing in meiosis.
KeywordsPolyploids Chromosome pairing Meiosis Chromosome painting
We thank Drs. Bernd Friebe and Tomás Naranjo for valuable comments on the manuscript.
J.J. conceived the research, L.H. and G.T.B. and conducted FISH experiments. L.H., G.T.B., G.A.T., and J.J. analyzed data. J.J. wrote the article.
This research was supported partially by National Science Foundation (NSF) grant ISO-1237969 and MSU startup funds to J.J.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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