Chromosome identification for the carnivorous plant Genlisea margaretae
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Genlisea margaretae, subgenus Genlisea, section Recurvatae (184 Mbp/1C), belongs to a plant genus with a 25-fold genome size difference and an extreme genome plasticity. Its 19 chromosome pairs could be distinguished individually by an approach combining optimized probe pooling and consecutive rounds of multicolor fluorescence in situ hybridization (mcFISH) with bacterial artificial chromosomes (BACs) selected for repeat-free inserts. Fifty-one BACs were assigned to 18 chromosome pairs. They provide a tool for future assignment of genomic sequence contigs to distinct chromosomes as well as for identification of homeologous chromosome regions in other species of the carnivorous Lentibulariaceae family, and potentially of chromosome rearrangements, in cases where more than one BAC per chromosome pair was identified.
KeywordsGenlisea Multicolor fluorescence in situ hybridization (mcFISH) Reprobing BACs Karyotyping
We thank Ines Walde and Kristin Langanke for technical assistance; Tomáš Beseda, Jan Vrána, and Jana Dostálová for assistance with flow sorting and BAC library construction; and Giang T.H. Vu and Hieu X. Cao for helpful discussions. This work was supported by a grant of the Deutsche Forschungsgemeinschaft to IS and JF (SCHU 951/16-1), by the European Social Fund (CZ.1.07/2.3.00/20.0189) to IS, by the Ministry of Education, Youth and Sports of the Czech Republic (grant LO1204 from the National Program of Sustainability I) to HŠ and JD, and by a PhD scholarship of the Vietnam Ministry of Education and Training to TDT.
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no competing interests.
- Bennett MD, Leitch IJ, Price HJ, Johnston JS (2003) Comparisons with Caenorhabditis (approximately 100 Mb) and Drosophila (approximately 175 Mb) using flow cytometry show genome size in Arabidopsis to be approximately 157 Mb and thus approximately 25% larger than the Arabidopsis genome initiative estimate of approximately 125 Mb. Ann Bot 91:547–557CrossRefPubMedPubMedCentralGoogle Scholar
- Betekhtin A, Jenkins G, Hasterok R (2014) Reconstructing the evolution of Brachypodium genomes using comparative chromosome painting. Plos One 9 doi: 10.1371/journal.pone.0115108
- Fleischmann A, Schaeferhoff B, Heubl G, Rivadavia F, Barthlott W, Mueller K (2010) Phylogenetics and character evolution in the carnivorous plant genus Genlisea A. St.-Hil. (Lentibulariaceae). Mol Phylogen Evol:768-783.Google Scholar
- Fleischmann A, Michael TP, Rivadavia F, Sousa A, Wang W, Temsch EM, Greilhuber J, Müller KF, Heubl G (2014) Evolution of genome size and chromosome number in the carnivorous plant genus Genlisea (Lentibulariaceae), with a new estimate of the minimum genome size in angiosperms. Ann Bot 114:1651–1663. doi: 10.1093/aob/mcu189 CrossRefPubMedPubMedCentralGoogle Scholar
- Green MR, Sambrook J (2012) Molecular cloning: a laboratory manual (Fourth edition) vol 1. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- Hasterok R, Marasek A, Donnison IS, Armstead I, Thomas A, King IP, Wolny E, Idziak D, Draper J, Jenkins G (2006a) Alignment of the genomes of Brachypodium distachyon and temperate cereals and grasses using bacterial artificial chromosome landing with fluorescence in situ hybridization. Genetics 173:349–362. doi: 10.1534/genetics.105.049726 CrossRefPubMedPubMedCentralGoogle Scholar
- Janda J, Šafář J, Kubaláková M, Bartoš J, Kovářová P, Suchánková P, Pateyron S, Číhalíková J, Sourdille P, Šimková H, Faivre-Rampant P, Hřibová E, Bernard M, Lukaszewski A, Doležel J, Chalhoub B (2006) Advanced resources for plant genomics: BAC library specific for the short arm of wheat chromosome 1B. Plant J 47:977–986. doi: 10.1111/j.1365-313X.2006.02840.x CrossRefPubMedGoogle Scholar
- Lysak MA, Mandakova T, Schranz ME (2016) Comparative paleogenomics of crucifers: ancestral genomic blocks revisited. Curr Opin Plant Biol 30:108-115. doi: 10.1016/j.pbi.2016.02.001Google Scholar
- Tran TD, Cao HX, Jovtchev G, Neumann P, Novák P, Fojtová M, Vu GT, Macas J, Fajkus J, Schubert I, Fuchs J (2015a) Centromere and telomere sequence alterations reflect the rapid genome evolution within the carnivorous plant genus Genlisea. Plant J 84:1087–1099. doi: 10.1111/tpj.13058 CrossRefPubMedGoogle Scholar
- Vu GTH, Schmutzer T, Bull F, Cao HX, Fuchs J, Tran TD, Jovtchev G, Pistrick K, Stein N, Pecinka A, Neumann P, Novak P, Macas J, Dear PH, Blattner FR, Scholz U, Schubert I (2015) Comparative genome analysis reveals divergent genome size evolution in a carnivorous plant genus. Plant Genome 8:3. doi: 10.3835/plantgenome2015.04.0021 CrossRefGoogle Scholar
- Wang K, Guan B, Guo W, Zhou B, Hu Y, Zhu Y, Zhang T (2008) Completely distinguishing individual A-genome chromosomes and their karyotyping analysis by multiple bacterial artificial chromosome—fluorescence in situ hybridization. Genetics 178:1117–1122. doi: 10.1534/genetics.107.083576 CrossRefPubMedPubMedCentralGoogle Scholar
- Yang L, Koo DH, Li D, Zhang T, Jiang J, Luan F, Renner SS, Hénaff E, Sanseverino W, Garcia-Mas J, Casacuberta J, Senalik DA, Simon PW, Chen J, Weng Y (2014) Next-generation sequencing, FISH mapping and synteny-based modeling reveal mechanisms of decreasing dysploidy in Cucumis. Plant J 77:16–30. doi: 10.1111/tpj.12355