Evolution of chromosome 6 of Solanum species revealed by comparative fluorescence in situ hybridization mapping
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Comparative genetic linkage mapping using a common set of DNA markers in related species is an important methodology in plant genome research. Here, we demonstrate a comparative fluorescence in situ hybridization (FISH) mapping strategy in plants. A set of 13 bacterial artificial chromosome clones spanning the entire length of potato chromosome 6 was used for pachytene chromosome-based FISH mapping in seven distantly related Solanum species including potato, tomato, and eggplant. We discovered one paracentric inversion and one pericentric inversion within specific lineages of these species. The comparative FISH mapping data revealed the ancestral structure of this chromosome. We demonstrate that comparative FISH mapping is an efficient and powerful methodology to study chromosomal evolution among plant species diverged for up to 12 million years.
KeywordsBacterial Artificial Chromosome Bacterial Artificial Chromosome Clone Solanum Species Pachytene Chromosome Paracentric Inversion
This work was supported by grant DBI-0604907 from the National Science Foundation. Q.F.L. was supported by a fellowship from the Ministry of Education, the People’s Republic of China. D.M.S. was supported by the USDA and by NSF DEB 0316614 and USDA National Research Initiative Grant 2008-35300-18669. M.I. was on leave of absence from CNR—Institute of Plant Genetics, Via Amendola 165/A, 70126 Bari, Italy.
- Amarillo FIE, Bass HW (2007) A transgenomic cytogenetic sorghum (Sorghum propinquum) bacterial artificial chromosome fluorescence in situ hybridization map of maize (Zea mays L.) pachytene chromosome 9, evidence for regions of genome hyperexpansion. Genetics 177:1509–1526CrossRefPubMedGoogle Scholar
- Cheng ZK, Presting GG, Buell CR, Wing RA, Jiang JM (2001) High-resolution pachytene chromosome mapping of bacterial artificial chromosomes anchored by genetic markers reveals the centromere location and the distribution of genetic recombination along chromosome 10 of rice. Genetics 157:1749–1757PubMedGoogle Scholar
- Howell EC, Armstrong SJ, Barker GC, Jones GH, King GJ, Ryder CD, Kearsey MJ (2005) Physical organization of the major duplication on Brassica oleracea chromosome O6 revealed through fluorescence in situ hybridization with Arabidopsis and Brassica BAC probes. Genome 48:1093–1103CrossRefPubMedGoogle Scholar
- Jackson SA, Cheng ZK, Wang ML, Goodman HM, Jiang J (2000) Comparative fluorescence in situ hybridization mapping of a 431-kb Arabidopsis thaliana bacterial artificial chromosome contig reveals the role of chromosomal duplications in the expansion of the Brassica rapa genome. Genetics 156:833–838PubMedGoogle Scholar
- Matsubayashi M (1991) Phylogenetic relationships in the potato and its related species. In: Tsuchiya T, Gupta P (eds) Chromosome engineering in plants: genetics, breeding, evolution. Elsevier, Amsterdam, pp 93–118Google Scholar
- Olmstead RG, Bohs L (2006) A summary of molecular systematic research in the Solanaceae: 1982–2006. Acta Hort 745:255–268Google Scholar
- Tang XM, Szinay D, Lang C, Ramanna MS, van der Vossen EAG, Datema E, Lankhorst RK, de Boer J, Peters SA, Bachem C, Stiekema W, Visser RGF, de Jong H, Bai YL (2008) Cross-species bacterial artificial chromosome-fluorescence in situ hybridization painting of the tomato and potato chromosome 6 reveals undescribed chromosomal rearrangements. Genetics 180:1319–1328CrossRefPubMedGoogle Scholar
- Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Roder MS, Wing RA, Wu W, Young ND (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160PubMedGoogle Scholar