Evolution of chromosome 6 of Solanum species revealed by comparative fluorescence in situ hybridization mapping
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Comparative genetic linkage mapping using a common set of DNA markers in related species is an important methodology in plant genome research. Here, we demonstrate a comparative fluorescence in situ hybridization (FISH) mapping strategy in plants. A set of 13 bacterial artificial chromosome clones spanning the entire length of potato chromosome 6 was used for pachytene chromosome-based FISH mapping in seven distantly related Solanum species including potato, tomato, and eggplant. We discovered one paracentric inversion and one pericentric inversion within specific lineages of these species. The comparative FISH mapping data revealed the ancestral structure of this chromosome. We demonstrate that comparative FISH mapping is an efficient and powerful methodology to study chromosomal evolution among plant species diverged for up to 12 million years.
KeywordsBacterial Artificial Chromosome Bacterial Artificial Chromosome Clone Solanum Species Pachytene Chromosome Paracentric Inversion
This work was supported by grant DBI-0604907 from the National Science Foundation. Q.F.L. was supported by a fellowship from the Ministry of Education, the People’s Republic of China. D.M.S. was supported by the USDA and by NSF DEB 0316614 and USDA National Research Initiative Grant 2008-35300-18669. M.I. was on leave of absence from CNR—Institute of Plant Genetics, Via Amendola 165/A, 70126 Bari, Italy.
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