Chromosoma

, Volume 119, Issue 3, pp 227–241

SUV39h-independent association of HP1β with fibrillarin-positive nucleolar regions

  • Andrea Harničarová Horáková
  • Eva Bártová
  • Gabriela Galiová
  • Radka Uhlířová
  • Pavel Matula
  • Stanislav Kozubek
RESEARCH ARTICLE

Abstract

Heterochromatin protein 1 (HP1), which binds to sites of histone H3 lysine 9 (H3K9) methylation, is primarily responsible for gene silencing and the formation of heterochromatin. We observed that HP1β is located in both the chromocenters and fibrillarin-positive nucleoli interiors. However, HP1α and HP1γ occupied fibrillarin-positive compartments to a lesser extent, corresponding to the distinct levels of HP1 subtypes at the promoter of rDNA genes. Deficiency of histone methyltransferases SUV39h and/or inhibition of histone deacetylases (HDACi) decreased HP1β and H3K9 trimethylation at chromocenters, but not in fibrillarin-positive regions that co-localized with RNA polymerase I. Similarly, SUV39h- and HDACi-dependent nucleolar rearrangement and inhibition of rDNA transcription did not affect the association between HP1β and fibrillarin. Moreover, the presence of HP1β in nucleoli is likely connected with transcription of ribosomal genes and with the role of fibrillarin in nucleolar processes.

Abbreviations

μl

Microliter(s)

μm

Micrometer(s)

3D

Three-dimensional

BSA

Bovine serum albumin

CD

Chromodomain

CSD

C-terminal chromoshadow domain

DMEM

Dulbecco's modified Eagle's medium

dn

Double null

Dnmt

DNA methyltransferase

FITC

Fluorescein isothiocyanate

GFP

Green fluorescence protein

h

Hour

H3

Histone H3

H1, H2A, H2B, H4

histones H1, H2A, H2B, H4

HDACs

Histone deacetylases

HMTs

Histone methyltransferases

HP1

Heterochromatin protein 1

K (K4, K9, K27...)

Lysine

MEFs

Mouse embryonic fibroblasts

MeCP2

Methyl CpG-binding protein

PBS

Phosphate-buffered saline

PML

Promyelocytic leukemia bodies

RT

Room temperature

RNA Pol I

RNA polymerase I

snoRNAs

Small nucleolar RNAs

wt

Wild type

TSA

Trichostatin A

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Andrea Harničarová Horáková
    • 1
  • Eva Bártová
    • 1
  • Gabriela Galiová
    • 1
  • Radka Uhlířová
    • 1
  • Pavel Matula
    • 2
  • Stanislav Kozubek
    • 1
  1. 1.Institute of BiophysicsAcademy of Sciences of the Czech RepublicBrnoCzech Republic
  2. 2.Faculty of InformaticsMasaryk UniversityBrnoCzech Republic

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