A typical way of moving chromosomes is exemplified by mitotic segregation, in which the centromere is directly captured by spindle microtubules. In this study, we highlight another way of moving chromosomes remotely from outside the nucleus, which involves SUN and KASH domain nuclear envelope proteins. SUN and KASH domain protein families are known to connect the nucleus to cytoskeletal networks and play a role in migration and positioning of the nucleus. Recent studies in the fission yeast Schizossacharomyces pombe demonstrated an additional role for the SUN–KASH protein complex in chromosome movements. During meiotic prophase, telomeres are moved to rearrange chromosomes within the nucleus. The SUN–KASH protein complex located in the nuclear envelope is involved in this process. Telomeres are connected to the SUN protein on the nucleoplasmic side, and the dynein motor complex binds to the KASH protein on the cytoplasmic side. Telomeres are then moved along the nuclear envelope using cytoplasmic microtubules. These findings illustrate a general mechanism for transmitting a cytoskeletal driving force to chromosomes across the nuclear envelope.
KeywordsNuclear Envelope Fission Yeast Meiotic Prophase Emerin Mitotic Cell Cycle
We would like to thank Abby F. Dernburg, Michael E. Dresser, and Akira Shinohara for providing their results before the publication. This work was supported by grants from the Japanese Ministry MEXT and the Japan Science and Technology Agency.
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- Cooper JP, Hiraoka Y (2006) Fission yeast telomere. In: de Lange T, Lundblad V, Blackburn E (eds) Telomeres, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 495–523Google Scholar
- Ding X, Xu R, Yu J, Xu T, Zhuang Y, Han M (2007) SUN1 is required for telomere attachment to nuclear envelope and gametogenesis in mice. Dev Cell 12:863–872Google Scholar
- Fridkin A, Mills E, Margalit A, Neufeld E, Lee KK, Feinstein N, Cohen M, Wilson KL, Gruenbaum Y (2004) Matefin, a Caenorhabditis elegans germ line-specific SUN-domain nuclear membrane protein, is essential for early embryonic and germ cell development. Proc Natl Acad Sci USA 101:6987–6992PubMedCrossRefGoogle Scholar
- Malone CJ, Fixsen WD, Horvitz HR, Han M (1999) UNC-84 localizes to the nuclear envelope and is required for nuclear migration and anchoring during C. elegans development. Development 126:3171–3181Google Scholar
- Zhang Q, Ragnauth CD, Skepper JN, Worth NF, Warren DT, Roberts RG, Weissberg PL, Ellis JA, Shanahan CM (2005) Nesprin-2 is a multi-isomeric protein that binds lamin and emerin at the nuclear envelope and forms a subcellular network in skeletal muscle. J Cell Sci 118:673–687PubMedCrossRefGoogle Scholar