, Volume 114, Issue 1, pp 54–66 | Cite as

Chriz, a chromodomain protein specific for the interbands of Drosophila melanogaster polytene chromosomes

  • A. A. Gortchakov
  • H. Eggert
  • M. Gan
  • J. Mattow
  • I. F. Zhimulev
  • H. Saumweber
Research Article


Polytene interphase chromosomes are compacted into a series of bands and interbands reflecting their organization into independent chromosomal domains. In order to understand chromosomal organization, we set out to study the role of proteins that are selective for interbands. Here we describe the Drosophila melanogaster chromodomain protein Chriz that is coimmunoprecipitated with the zinc finger protein Z4. Both proteins colocalize exclusively to the interbands on Drosophila polytene chromosomes. Like Z4, Chriz is ubiquitously expressed throughout development and is associated with chromatin in all interphase nuclei. Following dissociation from chromatin, early in mitosis Chriz binds to the centrosomes and to the mitotic spindle. Newly induced amorphic Chriz alleles are early lethal, and ubiquitous overexpression of Chriz is lethal as well. Available Chriz hypomorphs which survive until pupal stage have a normal chromosomal phenotype. Reducing Z4 protein does not affect Chriz binding to polytene chromosomes and vice versa. Z4 is still chromosomally bound when Chriz protein is depleted by RNA interference.



The authors thank I. Passow and R. Gienapp for excellent technical assistance. We acknowledge the help of K. Matthews from Bloomington Stock Center for provision of fly stocks and A. Carpenter for her kind gift of the transposase donor stock. I.F.Z. and A.A.G. were supported by a short-term DAAD Scholarship, by Programs for Molecular and Cellular Biology 10.1 and N70/2004, Program for Scientific Schools 918.2003, and by a grant Frontiers in Genetics 2-04. H.E. and H.S. were supported by a grant from DFG Sa338/9-1 Schwerpunkt Epigenetics.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • A. A. Gortchakov
    • 1
  • H. Eggert
    • 2
  • M. Gan
    • 2
  • J. Mattow
    • 3
  • I. F. Zhimulev
    • 1
  • H. Saumweber
    • 2
  1. 1.Institute of Cytology and GeneticsSiberian Branch of Russian Academy of SciencesNovosibirskRussia
  2. 2.Cytogenetics Division, Institute of BiologyHumboldt UniversityBerlinGermany
  3. 3.Max Planck Institute for Infection BiologyBerlinGermany

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