, Volume 113, Issue 8, pp 428–439 | Cite as

Identification and dynamics of Cajal bodies in relation to karyosphere formation in scorpionfly oocytes

  • F. M. Batalova
  • I. S. Stepanova
  • I. N. Skovorodkin
  • D. S. Bogolyubov
  • V. N. Parfenov
Research Article


In oocyte nuclei of the scorpionfly, Panorpa communis, we have recently defined a population of nuclear bodies (NBs) that contain some components of Cajal bodies (CBs). In the present study, we used several criteria [presence of coilin, U7 snRNA, RNA polymerase II (pol II) and specific ultrastructure] to identify these NBs as CBs. The essential evidence for CB identification came from experiments with microinjection of fluorescein-tagged U7 snRNA. Consistent with the U7 data, we found pol II and pre-mRNA splicing factor, SC35, in Panorpa oocyte CBs. We show here that the dynamics of CBs differs from that in somatic cells and correlates with the level of oocyte chromosome condensation. We also found that the significant increase of CB size is accompanied by condensation of the chromosomes in the karyosphere, which is indicative of a decline in transcription. Using immunogold microscopy we determined that pol II and coilin are shared by CBs and the granular material associated with condensed chromosomes in the Panorpa karyosphere. The colocalization of pol II, U7 snRNA and splicing factors with CBs at the inactive stage of late oogenesis suggests that the latter may serve as storage domains for components that were earlier engaged in RNA transcription and processing.



We are grateful to the following people for providing antibodies used in this work: E. K. L. Chan for R288 serum, K. G. Murti for the anti-DNA monoclonal antibody, J. G. Gall for mAbs 8WG16 and anti-SC35. The authors are greatly indebted to J. G. Gall for providing fluorescein-tagged RNA constructs. We also thank Yu. I. Gukina for technical assistance. This work was supported by the Russian Foundation for Basic Research (grants No. 03-04-49389 and 04-04-48080) and the Russian Science Support Foundation.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • F. M. Batalova
    • 1
  • I. S. Stepanova
    • 1
  • I. N. Skovorodkin
    • 1
  • D. S. Bogolyubov
    • 1
  • V. N. Parfenov
    • 1
  1. 1.Laboratory of Cell MorphologyInstitute of Cytology, Russian Academy of SciencesSt PetersburgRussia

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