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Endoreduplication in Drosophila melanogaster progeny after exposure to acute γ-irradiation

  • Daria A. Skorobagatko
  • Alexey A. Mazilov
  • Volodymyr Yu. StrashnyukEmail author
Original Article

Abstract

The purpose of this investigation was to study the effect of acute γ-irradiation of parent adults on the endoreduplication of giant chromosomes in F1 generation of Drosophila melanogaster Meig. A wild-type Oregon-R strain was used as the material. Virgin females and males of Drosophila adults at the age of 3 days were irradiated with doses of 8, 16 and 25 Gy. Giant chromosomes were studied by cytomorphometry on squashed preparations of Drosophila salivary glands stained with acetoorsein. The preparations were obtained at late third instar larvae. The mean values of the polyteny degree of chromosomes (PDC) in males increased after 8 Gy by 10.6%, after 25 Gy by 7.4%, and did not change after the dose of 16 Gy. In females, the PDC did not differ from the control irrespective of the irradiation dose. An increase in endoreduplication was also evidenced by the accelerated development of offsprings of both sexes after irradiation of parents with 25 Gy, and in males also at a dose of 16 Gy. The statistical impact of power of radiation on polyteny was 26.8%, while the impact of sex was 4.9%. The impact of power of radiation on the developmental rate of offspring was 4.4% in males and 7.5% in females. The enhancement of endoreduplication is considered as a consequence of increasing selection pressure after irradiation. The possible involvement of epigenetic effects in the effect of ionizing radiation on endoreduplication is discussed.

Keywords

Giant chromosomes Polyteny degree Developmental rate Embryonic mortality Ionizing radiation 

Notes

Acknowledgements

This work was supported by the Ministry of Education and Science of Ukraine (Project State registration number: 0117U004836).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Genetics and CytologyVN Karazin Kharkiv National UniversityKharkivUkraine
  2. 2.Laboratory of Radiation Research and Environmental ProtectionNSC ‘Kharkiv Institute of Physics and Technology’KharkivUkraine

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