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Assessment of absorbed dose of gamma rays using the simultaneous determination of inactive hemoglobin derivatives as a biological dosimeter

  • A. M. M. Attia
  • W. M. Aboulthana
  • G. M. HassanEmail author
  • E. Aboelezz
Original Article
  • 52 Downloads

Abstract

Biological dosimetry based on sulfhemoglobin (SHb), methemoglobin (MetHb), and carboxyhemoglobin (HbCO) levels was evaluated. SHb, MetHb and HbCO levels were estimated in erythrocytes of mice irradiated by γ rays from a 60Co source using the method of multi-component spectrophotometric analysis developed recently. In this method, absorption measurements of diluted aqueous Hb-solution were made at λ = 500, 569, 577 and 620 nm, and using the mathematical formulas based on multi-component spectrophotometric analysis and the mathematical Gaussian elimination method for matrix calculation, the concentrations of various Hb-derivatives and total Hb in mice blood were estimated. The dose range of γ rays was from 0.5 to 8 Gy and the dose rate was 0.5 Gy min−1. Among all Hb-derivatives, MetHb, SHb and HbCO demonstrated an unambiguous dose-dependent response. For SHb and MetHb, the detection limits were about 0.5 Gy and 1 Gy, respectively. After irradiation, high levels of MetHb, SHb and HbCO persisted for at least 10 days, and the maximal increase of MetHb, SHb and HbCO occurred up to 24 h following γ irradiation. The use of this “MetHb + SHb + HbCO”-derivatives-based absorbed dose relationship showed a high accuracy. It is concluded that simultaneous determination of MetHb, SHb and HbCO, by multi-component spectrophotometry provides a quick, simple, sensitive, accurate, stable and inexpensive biological indicator for the early assessment of the absorbed dose in mice.

Keywords

Biological dosimetry γ-Irradiation Carboxyhemoglobin (HbCO) Methemoglobin (MetHb) Oxyhemoglobin (HbO2Sulfhemoglobin (SHb) 

Notes

Compliance with ethical standards

Conflict of interest

The authors, who are responsible for content and writing of the manuscript, have no declared conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • A. M. M. Attia
    • 1
  • W. M. Aboulthana
    • 1
  • G. M. Hassan
    • 2
    Email author
  • E. Aboelezz
    • 2
  1. 1.Genetic Engineering and Biotechnology Division, Biochemistry DepartmentNational Research CentreGizaEgypt
  2. 2.Division of Thermometry and Ionizing Radiation Metrology, Department of Ionizing Radiation MetrologyNational Institute of StandardsGizaEgypt

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