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Radiation and Environmental Biophysics

, Volume 58, Issue 3, pp 433–438 | Cite as

Selenium does not affect radiosensitivity of breast cancer cell lines

  • Daniela SchillingEmail author
  • Birgit Herold
  • Stephanie E. Combs
  • Thomas E. Schmid
Original Article
  • 105 Downloads

Abstract

Supplementation with the antioxidant selenium is frequently performed in breast cancer patients to protect the normal tissue from radiation-induced side effects. However, concerns exist whether selenium also protects tumor cells from radiation-induced cell kill and thereby reduces the efficacy of radiotherapy. In this work, the effect of selenium administration on the radiosensitivity of breast cancer cells was evaluated in vitro. Physiological relevant selenium concentrations (70 and 140 µg/l) did not affect DNA double-strand breaks (γH2AX foci) after 4-Gy X-ray irradiation. Also apoptosis (caspase 3/7) after irradiation with 10 Gy was not influenced by selenium treatment in MDA-MB-231 and MCF7 cells. Most importantly, selenium supplementation did not impair the clonogenic survival of the breast cancer cell lines after irradiation (0, 2, 4, 6, 8 Gy). The data suggest that physiological relevant selenium concentrations administered in combination with radiation therapy do not deteriorate the efficacy of radiotherapy in breast cancer patients. However, randomized clinical trials comparing the effectiveness of radiotherapy and the associated side effects in patients with and without selenium supplementation are recommended.

Keywords

Selenium Radiation Breast cancer Radiosensitivity Antioxidant Supplementation 

Notes

Acknowledgements

The authors thank Andrea Mair and Marlon Stein for excellent technical assistance.

Funding

This research received no external funding.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Radiation OncologyTechnische Universität München, School of Medicine, Klinikum rechts der IsarMunichGermany
  2. 2.Department of Radiation Sciences (DRS), Institute of Radiation Medicine (IRM)Helmholtz Zentrum MünchenNeuherbergGermany
  3. 3.Deutsches Konsortium für Translationale Krebsforschung (DKTK), Partner Site MunichMunichGermany

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