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Radiation and Environmental Biophysics

, Volume 53, Issue 4, pp 677–693 | Cite as

Physiological changes and anti-oxidative responses of Arabidopsis plants after acute and chronic γ-irradiation

  • Eun Jeong Goh
  • Jin-Baek Kim
  • Wook-Jin Kim
  • Bo-Keun Ha
  • Sang Hoon Kim
  • Si–Yong Kang
  • Yong Weon Seo
  • Dong Sub KimEmail author
Original Paper

Abstract

To identify the effects of acute and chronic γ-irradiation in Arabidopsis plants, physiological responses and antioxidant-related gene expression were investigated. Seedlings were exposed to 200 Gy of γ-irradiation in acute manner for 1 or 24 h (A1 and A24) or in chronic manner for 1, 2, or 3 weeks (C1 W, C2 W, and C3 W). Plant height, silique number, and silique length in A1 and A24 irradiated plants were significantly reduced when compared to non-irradiated plants. Silique number decreased in response to both acute and chronic irradiation, except with the C3 W treatment, and the number of trichomes dramatically increased in A1 and C1 W. Electron spin resonance signal intensities increased in A1 and in all chronically irradiated plants, but decreased in the A24-treated plant. To investigate the effects of acute and chronic γ-irradiation on antioxidant enzymes, we examined activity of four antioxidant enzymes: catalase (CAT), peroxidase (POD), ascorbate peroxidase, and superoxide dismutase. In general, POD and CAT activities decreased in response to acute and chronic γ-irradiation. Oligonucleotide microarrays were used to investigate transcriptional changes after irradiation. Several genes related to reactive oxygen species signaling were up-regulated after acute and chronic exposure, including genes encoding heat shock factors, zinc finger proteins, NADPH oxidase, WRKY DNA-binding proteins, and calcium binding proteins. Taken together, our data indicate that the responses and activation of antioxidant systems prompted by irradiation exposure are dependent upon the γ-ray dose rate.

Keywords

Acute and chronic irradiation Physiological response Antioxidant enzymes Microarray 

Abbreviations

ESR

Electron spin resonance

CAT

Catalase

POD

Peroxidase

APX

Ascorbate peroxidase

SOD

Superoxide dismutase

DSB

Double strand break

SSB

Single strand break

HR

Homologous recombination

NHEJ

Non-homologous end joining

ROS

Reactive oxygen species

TEM

Transmission electron microscopy

MDA

Malondialdehyde

TCA

Trichloroacetic acid

TBA

Thiobarbituric acid

POBN

α-(4-pyridyl-1-oxide)-N-tert-butylnitrone

HSF

Heat shock factor

Notes

Acknowledgments

This work was supported by grants from the Korea Atomic Energy Research Institute (KAERI) and the Nuclear R and D Program by the Ministry of Science, ICT and Future Planning (MSIP), Republic of Korea.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Eun Jeong Goh
    • 1
  • Jin-Baek Kim
    • 1
    • 3
  • Wook-Jin Kim
    • 2
  • Bo-Keun Ha
    • 4
  • Sang Hoon Kim
    • 1
  • Si–Yong Kang
    • 1
  • Yong Weon Seo
    • 3
  • Dong Sub Kim
    • 1
    Email author
  1. 1.Advanced Radiation Technology InstituteKorea Atomic Energy Research InstituteJeongeupRepublic of korea
  2. 2.Basic Herbal Medicine Research Group, Herbal Medicine Research DivisionKorea Institute of Oriental MedicineDaejeonRepublic of korea
  3. 3.Division of Biotechnology and Genetic EngineeringKorea UniversitySeoulRepublic of korea
  4. 4.Division of Plant BiotechnologyChonnam National UniversityGwangjuRepublic of korea

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