UV microspot irradiator at Columbia University
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The Radiological Research Accelerator Facility at Columbia University has recently added a UV microspot irradiator to a microbeam irradiation platform. This UV microspot irradiator applies multiphoton excitation at the focal point of an incident laser as the source for cell damage, and with this approach, a single cell within a 3D sample can be targeted and exposed to damaging UV. The UV microspot’s ability to impart cellular damage within 3D is an advantage over all other microbeam techniques, which instead impart damage to numerous cells along microbeam tracks. This short communication is an overview, and a description of the UV microspot including the following applications and demonstrations of selective damage to live single cell targets: DNA damage foci formation, patterned irradiation, photoactivation, targeting of mitochondria, and targeting of individual cardiomyocytes in a live zebrafish embryo.
KeywordsDNA damage Microbeams UV
The authors thank: Asithambi Aroumougame from the David Chen group at UTSW for providing GFP-tagged XRCC1 HT1080 cells and EGFP-tagged OGG1 HT1080 cells; Iris Muller at GSI and Jonathan Chubb at the University of Dundee for providing a triple-tagged HT1080 cell line (RFP, YFP, and photoactivable GFP); and Michael Kruhlak at NIH/NCI for providing HeLa cells with photoactivable GFP. The authors also thank Columbia University employees Helen Turner for preparing and handling HT1080 cells, Vanessa George and Manuela Buonanno for preparing and handling the zebrafish embryo samples, and Gary Johnson for instrumentation development. This work was supported by the National Institute of Biomedical Imaging and Bioengineering under Grant: NIBIB 5P41 EB002033-16.