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Radiation and Environmental Biophysics

, Volume 51, Issue 3, pp 283–293 | Cite as

Micronuclei in human peripheral blood lymphocytes exposed to mixed beams of X-rays and alpha particles

  • Elina Staaf
  • Karl Brehwens
  • Siamak Haghdoost
  • Sander Nievaart
  • Katerina Pachnerova-Brabcova
  • Joanna Czub
  • Janusz Braziewicz
  • Andrzej WojcikEmail author
Original Paper

Abstract

The purpose of this study was to analyse the cytogenetic effect of exposing human peripheral blood lymphocytes (PBL) to a mixed beam of alpha particles and X-rays. Whole blood collected from one donor was exposed to different doses of alpha particles (241Am), X-rays and a combination of both. All exposures were carried out at 37 °C. Three independent experiments were performed. Micronuclei (MN) in binucleated PBL were scored as the endpoint. Moreover, the size of MN was measured. The results show that exposure of PBL to a mixed beam of high and low linear energy transfer radiation led to significantly higher than expected frequencies of MN. The measurement of MN size did not reveal any differences between the effect of alpha particles and mixed beam. In conclusion, a combined exposure of PBL to alpha particles and X-rays leads to a synergistic effect as measured by the frequency of MN. From the analysis of MN distributions, we conclude that the increase was due to an impaired repair of X-ray-induced DNA damage.

Keywords

Micronuclei Combined exposure LET Mixed beams Alpha particles X-rays 

Notes

Acknowledgments

The study was supported by an exploratory research project from the Joint Research Centre, Institute of Energy, and by a grant from the Swedish Radiation Safety Authority (SSM).

Ethical Standards

The work was approved by the local ethical committee at the Karolinska University Hospital, Stockholm, Sweden (diarium number 2010/27-31/1).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Elina Staaf
    • 1
  • Karl Brehwens
    • 1
  • Siamak Haghdoost
    • 1
  • Sander Nievaart
    • 2
  • Katerina Pachnerova-Brabcova
    • 3
    • 4
  • Joanna Czub
    • 5
  • Janusz Braziewicz
    • 5
  • Andrzej Wojcik
    • 1
    • 5
    • 6
    Email author
  1. 1.Stockholms UniversitetStockholmSweden
  2. 2.Institute for Energy-JRCPettenThe Netherlands
  3. 3.Department of Radiation DosimetryNuclear Physics Institute, AS CRPragueCzech Republic
  4. 4.Department of Nuclear Chemistry, Faculty of Nuclear Sciences and EngineeringCzech Technical UniversityPragueCzech Republic
  5. 5.Jan Kochanowski UniversityKielcePoland
  6. 6.GMT Department, Centre for Radiation Protection ResearchStockholm UniversityStockholmSweden

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