Radiation and Environmental Biophysics

, Volume 50, Issue 1, pp 155–166

Low-dose irradiation causes rapid alterations to the proteome of the human endothelial cell line EA.hy926

  • Franka Pluder
  • Zarko Barjaktarovic
  • Omid Azimzadeh
  • Simone Mörtl
  • Anne Krämer
  • Sylvia Steininger
  • Hakan Sarioglu
  • Dariusz Leszczynski
  • Reetta Nylund
  • Arvi Hakanen
  • Arundhathi Sriharshan
  • Michael J. Atkinson
  • Soile Tapio
Original Paper

Abstract

High doses of ionising radiation damage the heart by an as yet unknown mechanism. A concern for radiological protection is the recent epidemiological data indicating that doses as low as 100–500 mGy may induce cardiac damage. The aim of this study was to identify potential molecular targets and/or mechanisms involved in the pathogenesis of low-dose radiation-induced cardiovascular disease. The vascular endothelium plays a pivotal role in the regulation of cardiac function and is therefore a potential target tissue. We report here that low-dose radiation induced rapid and time-dependent changes in the cytoplasmic proteome of the human endothelial cell line EA.hy926. The proteomes were investigated at 4 and 24 h after irradiation at two different dose rates (Co-60 gamma ray total dose 200 mGy; 20 mGy/min and 190 mGy/min) using 2D-DIGE technology. Differentially expressed proteins were identified, after in-gel trypsin digestion, by MALDI-TOF/TOF tandem mass spectrometry, and peptide mass fingerprint analyses. We identified 15 significantly differentially expressed proteins, of which 10 were up-regulated and 5 down-regulated, with more than ± 1.5-fold difference compared with unexposed cells. Pathways influenced by the low-dose exposures included the Ran and RhoA pathways, fatty acid metabolism and stress response.

Abbreviations

D-MEM

Dulbecco’s Modified Eagle’s Medium

PMMA

Poly(methyl methacrylate)

HAT

Hypoxanthine Aminopterin Thymidine

SNP

Single nucleotide polymorphism

Gy

Gray

o/n

Overnight

TFA

Trifluoroacetic acid

Supplementary material

411_2010_342_MOESM1_ESM.pdf (244 kb)
Supplementary material 1 (PDF 243 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Franka Pluder
    • 1
  • Zarko Barjaktarovic
    • 1
  • Omid Azimzadeh
    • 1
  • Simone Mörtl
    • 1
  • Anne Krämer
    • 1
  • Sylvia Steininger
    • 1
  • Hakan Sarioglu
    • 2
  • Dariusz Leszczynski
    • 3
  • Reetta Nylund
    • 3
  • Arvi Hakanen
    • 3
  • Arundhathi Sriharshan
    • 4
  • Michael J. Atkinson
    • 1
    • 5
  • Soile Tapio
    • 1
  1. 1.Institute of Radiation BiologyHelmholtz Zentrum München, German Research Center for Environmental HealthNeuherbergGermany
  2. 2.Department of Protein ScienceHelmholtz Zentrum München, German Research Center for Environmental HealthNeuherbergGermany
  3. 3.STUK—Radiation and Nuclear Safety AuthorityHelsinkiFinland
  4. 4.Division of Radiation CytogeneticsHelmholtz Zentrum München, German Research Center for Environmental HealthNeuherbergGermany
  5. 5.Department of Radiation OncologyKlinikum Rechts der Isar, Technische Universität MünchenMunichGermany

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