Radiation and Environmental Biophysics

, Volume 49, Issue 3, pp 437–445 | Cite as

Dendro[C60]fullerene DF-1 provides radioprotection to radiosensitive mammalian cells

  • Corey A. Theriot
  • Rachael C. Casey
  • Valerie C. Moore
  • Linsey Mitchell
  • Julia O. Reynolds
  • Madeline Burgoyne
  • Ranga Partha
  • Janice L. Huff
  • Jodie L. Conyers
  • Antony Jeevarajan
  • Honglu Wu
Original Paper

Abstract

In this study, the ability of the C60 fullerene derivative DF-1 to protect radiosensitive cells from the effects of high doses of gamma irradiation was examined. Earlier reports of DF-1’s lack of toxicity in these cells were confirmed, and DF-1 was also observed to protect both human lymphocytes and rat intestinal crypt cells against radiation-induced cell death. We determined that DF-1 protected both cell types against radiation-induced DNA damage, as measured by inhibition of micronucleus formation. DF-1 also reduced the levels of reactive oxygen species in the crypt cells, a unique capability of fullerenes because of their enhanced reactivity toward electron-rich species. The ability of DF-1 to protect against the cytotoxic effects of radiation was comparable to that of amifostine, another ROS-scavenging radioprotector. Interestingly, localization of fluorescently labeled DF-1 in fibroblast was observed throughout the cell. Taken together, these results suggest that DF-1 provides powerful protection against several deleterious cellular consequences of irradiation in mammalian systems including oxidative stress, DNA damage, and cell death.

Abbreviations

DCFH-DA

2′,7′-Dichlorofluoroscein diacetate

DF-1

Dendro[C60]fullerene-1

f-DF-1

Fluorescently labeled df-1

FBS

Fetal bovine serum

PHA

Phytohemagglutinin

PBS

Phosphate buffered saline

ROS

Reactive oxygen species

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Copyright information

© US Government 2010

Authors and Affiliations

  • Corey A. Theriot
    • 1
  • Rachael C. Casey
    • 2
  • Valerie C. Moore
    • 3
  • Linsey Mitchell
    • 3
  • Julia O. Reynolds
    • 4
  • Madeline Burgoyne
    • 4
  • Ranga Partha
    • 3
  • Janice L. Huff
    • 2
  • Jodie L. Conyers
    • 3
  • Antony Jeevarajan
    • 1
  • Honglu Wu
    • 1
  1. 1.NASA—Johnson Space CenterHoustonUSA
  2. 2.Universities Space Research AssociationHoustonUSA
  3. 3.University of Texas Health Science Center at HoustonHoustonUSA
  4. 4.Centenary College of LouisianaShreveportUSA

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