Modeling radiation-induced cell cycle delays
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Ionizing radiation is known to delay the cell cycle progression. In particular after particle exposure significant delays have been observed and it has been shown that the extent of delay affects the expression of damage, such as chromosome aberrations. Thus, to predict how cells respond to ionizing radiation and to derive reliable estimates of radiation risks, information about radiation-induced cell cycle perturbations is required. In the present study we describe and apply a method for retrieval of information about the time-course of all cell cycle phases from experimental data on the mitotic index only. We study the progression of mammalian cells through the cell cycle after exposure. The analysis reveals a prolonged block of damaged cells in the G2 phase. Furthermore, by performing an error analysis on simulated data valuable information for the design of experimental studies has been obtained. The analysis showed that the number of cells analyzed in an experimental sample should be at least 100 to obtain a relative error <20%.
KeywordsCell Cycle Progression Mitotic Index Cell Cycle Phase Current Phase Duration Distribution
This work was supported in part (A. O-M) by the grant of Polish State Committee for Scientific Research (KBN, Grant No 1 P03B 159 29). Moreover, E. G-N acknowledges Marie Curie TOK COCOS grant at the Mark Kac Complex Systems Research Center in Kraków, Poland. E.N. was supported by BMBF (Bonn, Germany) under contract number 02S8203 and 02S8497.
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