Radiation and Environmental Biophysics

, Volume 48, Issue 2, pp 197–203 | Cite as

The effect of electron and gamma irradiation on the induction of micronuclei in cytokinesis-blocked human blood lymphocytes

  • Santhosh Acharya
  • Ganesh Sanjeev
  • N. N. Bhat
  • K. Siddappa
  • Y. Narayana
Original Paper

Abstract

The effect of electrons and gamma irradiation on the induction of micronuclei in cytokinesis-blocked human peripheral blood lymphocytes was investigated to understand the relative biological effectiveness (RBE) of electrons compared with gamma rays. Blood samples were irradiated with an 8 MeV pulsed electron beam, at a mean instantaneous dose rate of 2.6 × 105 Gy s−1. Gamma irradiation was carried out at a dose rate of 1.98 Gy min−1 using 60Co gamma source. A dose-dependent increase in micronuclei yield was observed. The dose–response relationships for induction of micronuclei fitted well to a linear–quadratic relationship and the coefficients α and β of the dose–response curve were estimated by fitting the data using error-weighted minimum χ2 method. The RBE of 8 MeV electrons were found to be near unity as compared with gamma rays.

Notes

Acknowledgment

The authors are grateful to research colleagues and technical staff at Microtron Centre, Mangalore University and Radiological Physics and Advisory Division (RP & AD), BARC, Mumbai, for their help and support during the course of work. The authors gratefully acknowledge the help and useful suggestions of Dr. B.S. Rao, Former Head, RP & AD, BARC, Mumbai. One of the authors (S.A.) is grateful to Board of Research in Nuclear Sciences, Department of Atomic Energy, Government of India for financial support.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Santhosh Acharya
    • 1
  • Ganesh Sanjeev
    • 1
  • N. N. Bhat
    • 2
  • K. Siddappa
    • 3
  • Y. Narayana
    • 1
  1. 1.Microtron Centre, Department of Studies in PhysicsMangalore UniversityMangaloreIndia
  2. 2.Radiological Physics and Advisory DivisionBhabha Atomic Research Centre (BARC)MumbaiIndia
  3. 3.JSS Foundation for Science and SocietyBangaloreIndia

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