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Radiation and Environmental Biophysics

, Volume 46, Issue 4, pp 409–416 | Cite as

Induction of DNA double-strand breaks by zeocin in Chlamydomonas reinhardtii and the role of increased DNA double-strand breaks rejoining in the formation of an adaptive response

  • S. G. ChankovaEmail author
  • E. Dimova
  • M. Dimitrova
  • P. E. Bryant
Original Paper

Abstract

This study aimed to test the potential of the radiomimetic chemical zeocin to induce DNA double-strand breaks (DSB) and “adaptive response” (AR) in Chlamydomonas reinhardtii strain CW15 as a model system. The AR was measured as cell survival using a micro-colony assay, and by changes in rejoining of DSB DNA. The level of induced DSB was measured by constant field gel electrophoresis based on incorporation of cells into agarose blocks before cell lysis. This avoids the risk of accidental induction of DSB during the manipulation procedures. Our results showed that zeocin could induce DSB in C. reinhardtii strain CW15 in a linear dose-response fashion up to 100 μg ml−1 which marked the beginning of a plateau. The level of DSB induced by 100 μg ml−1 zeocin was similar to that induced by 250 Gy of gamma-ray irradiation. It was also found that, similar to gamma rays, zeocin could induce AR measured as DSB in C. reinhardtii CW15 and this AR involved acceleration of the rate of DSB rejoining, too. To our knowledge, this is the first demonstration that zeocin could induce AR in some low eukaryotes such as C. reinhardtii.

Keywords

Zeocin Radiomimetic Gamma rays Genotoxicity Adaptive response DSB Chlamydomonas 

Notes

Acknowledgments

Zeocin (InvivoGen) and an electrophoresis camera (Amersham Biosciences) were kindly provided by D.Sc. P.E. Bryant, St Andrews University, Scotland. This work was supported by the Bulgarian Ministry of Education and Science (project K-1204, project BioCore), St Andrews University, The Royal Society, UK, and the Bulgarian Academy of Sciences.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • S. G. Chankova
    • 1
    Email author
  • E. Dimova
    • 1
  • M. Dimitrova
    • 1
  • P. E. Bryant
    • 2
  1. 1.Central Laboratory of General Ecology-BASSofiaBulgaria
  2. 2.Bute Medical SchoolUniversity of St AndrewsSt AndrewsUK

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