Protective Association of TIM1−1454G>A Polymorphism with Asthma in a North Indian Population
- 130 Downloads
TIM1 is a key regulator of Th2-dominated immune responses, including allergy, asthma, autoimmunity, and response to the pathogens. They are mainly expressed by hepatocytes and lymphoid cells. Analysis of the sequence of TIM1 was found to have range of SNPs which increases the transcriptional activity of the TIM1 gene.
A case–control study was conducted with a total of 964 subjects, including 483 healthy controls and 481 asthma patients in the present study. DNA samples were extracted from blood, and genotyping was done using polymerase chain reaction–restriction fragment length polymorphism method.
Statistical analysis revealed that both heterozygous (GA) as well as the mutant (AA) genotype of −1454G>A (rs41297579) polymorphism shows resistance toward asthma with OR = 0.74, 95 % CI (0.55–0.98), p = 0.029 and OR = 0.43, 95 % CI (0.28–0.65), p = 0.000, respectively. The mutant (A) allele was also found to be highly protective toward asthma with OR = 0.68, 95 % CI (0.56–0.82) p = 0.000. However, no statistical difference was found between the TIM1–416G>C (rs9313422) polymorphism and asthma patients (p > 0.05).
This is the first study conducted in India conferring −1454G>A polymorphism provides resistance toward asthma while lack of association was found between −416G>C polymorphism and asthma in the studied North Indian population.
KeywordsAsthma North India TIM1 Polymorphisms PCR–RFLP Total IgE
The authors thank the medical institute for their cooperation in allowing them to contact the patients and to obtain their detailed clinical information.
Conflict of interest
- 1.Cavkaytar O, Sekerel BE (2014) Baseline management of asthma control. Allergol Immunopathol (Madr) 42(2):162–168. doi:10.1016/j.aller.2012.10.004
- 12.Gao PS, Mathias RA, Plunkett B, Togias A, Barnes KC, Beaty TH et al (2005) Genetic variants of the T-cell immunoglobulin mucin 1 but not the T-cell immunoglobulin mucin 3 gene are associated with asthma in an African American population. J Allergy Clin Immunol 115(5):982–988PubMedCrossRefGoogle Scholar
- 15.Rathod VPS, Kapoor P, Pillai KK, Khanam R (2010) Assessment of asthma and chronic obstructive pulmonary disorder in relation to reversibility, IgE, eosinophil, and neutrophil count in a university teaching hospital in South Delhi, India. J Pharm Bioallied Sci 2(4):337–340. doi:10.4103/0975-7406.72136 PubMedCentralPubMedCrossRefGoogle Scholar
- 18.Roe BA, Crabtree JS, Khan AS (1996) DNA isolation and sequencing. In: Rickwood D (ed) Essential techniques series, Wiley, New York, pp 85–86, 116–117Google Scholar
- 24.Christodoulopoulos P, Cameron L, Nakamura Y, Lemiere C, Muro S et al (2001) Th2 cytokine-associated transcription factors in atopic and nonatopic asthma: evidence for differential signal transducer and activator of transcription 6 expression. J Allergy Clin Immunol 107(4):586–591PubMedCrossRefGoogle Scholar