, Volume 189, Issue 1, pp 57–63 | Cite as

Clinical Usefulness of D2-40 in Non-Small Cell Lung Cancer

  • Kyung Hoon Min
  • Seoung Ju Park
  • Kyung Sun Lee
  • Sung Ho Hwang
  • So Ri Kim
  • Hee Moon
  • Hyo Jin Han
  • Myoung Ja Chung
  • Yong Chul Lee


D2-40 is a recently developed monoclonal antibody that reacts with a 40 kDa O-linked sialoglycoprotein and has been used for the assessment of lymphatic invasion in tumor specimens. We have evaluated the diagnostic usefulness of D2-40 and association of its immunopositivity with clinicopathological parameters in adenocarcinoma and squamous cell carcinoma of the lung. We investigated 97 cases of surgically resected adenocarcinoma or squamous cell carcinoma of the lung for the determination of D2-40 positivity in tumor cells and peritumoral lymphatic vessel density (LVD) using an immunostaining method. D2-40 immunoreactivity in tumor cells was invariably negative in adenocarcinoma but 47% of squamous cell carcinomas were positive. D2-40 positivity in the tumor was significantly associated with high LVD in squamous cell carcinoma (P < 0.006). There was no significant association between peritumoral LVD and clinicopathologic parameters, including lymphatic vessel invasion, lymph node metastasis, and survival in adenocarcinoma and squamous cell carcinoma. These results suggest that D2-40 immunoreactivity in tumor cells can be used for distinguishing between adenocarcinoma and squamous cell carcinoma and that the reactivity of tumor cells with D2-40 is positively correlated with LVD in squamous cell carcinoma but not with lymph node metastasis in adenocarcinoma and squamous cell carcinoma.


D2-40 Lymphangiogenesis Lymphatic metastasis Lymphatic vessels Non-small cell lung cancer 



We thank professor Mie-Jae Im for careful reading of the manuscript. This study was supported by a grant from the Korea Healthcare Technology R&D Project, Ministry for Health and Welfare, Republic of Korea (A084144).




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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Kyung Hoon Min
    • 1
    • 3
  • Seoung Ju Park
    • 1
    • 3
  • Kyung Sun Lee
    • 1
    • 3
  • Sung Ho Hwang
    • 2
  • So Ri Kim
    • 1
    • 3
  • Hee Moon
    • 1
    • 3
  • Hyo Jin Han
    • 1
    • 3
  • Myoung Ja Chung
    • 2
    • 3
  • Yong Chul Lee
    • 1
    • 3
  1. 1.Department of Internal MedicineChonbuk National University Medical SchoolJeonjuSouth Korea
  2. 2.Department of PathologyChonbuk National University Medical SchoolJeonjuSouth Korea
  3. 3.Research Center for Pulmonary DisordersChonbuk National University HospitalJeonjuSouth Korea

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