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Lung

, 187:98 | Cite as

Ebselen Improves Ischemia-Reperfusion Injury After Rat Lung Transplantation

  • Jürg HamacherEmail author
  • Uz Stammberger
  • Elvira Weber
  • Rudolf Lucas
  • Albrecht Wendel
Article

Abstract

The heterocyclic organic compound ebselen (2-phenyl-1,2-benizsoselenazol-3(2H)-one) is a glutathione peroxidase mimick with protective properties against oxidative injury. Ebselen also has anti-inflammatory activity, including attenuation of tumor necrosis factor release and increase of interleukin-10, as shown in vivo, in inflammatory and ischemia-reperfusion injuries, including those of the lung. This study was designed to assess its effect on severe ischemia-reperfusion injury in a model of left-sided rat lung isotransplantation. Orthotopic single left-sided lung allotransplantation (Wistar to Wistar) was performed in female rats after a total ischemic time of 18 h. In nine recipients given 500 mg/kg oral ebselen 1 h before transplantation, graft PaO2/FiO2 was improved 24 h after transplantation, as evidenced with a mean (standard deviation) PaO2 of 139 (61) mmHg vs. eight controls with 65 (33) mmHg (p = 0.009). Bronchoalveolar PMN count was reduced to approximately 50% in the ebselen group compared with controls, whereas no difference in the tumor necrosis factor content was found. We conclude that the improvement of lung function in ebselen-treated transplanted rats is mainly the result of the anti-inflammatory activity of the drug during reperfusion.

Keywords

Ebselen Lung transplantation Reperfusion injury Anti-inflammatory agents 

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jürg Hamacher
    • 1
    • 2
    Email author
  • Uz Stammberger
    • 3
  • Elvira Weber
    • 1
  • Rudolf Lucas
    • 1
    • 4
  • Albrecht Wendel
    • 1
  1. 1.Biochemical PharmacologyUniversity of KonstanzKonstanzGermany
  2. 2.Pulmonary DivisionUniversity Hospital InselspitalBernSwitzerland
  3. 3.Division of General Thoracic SurgeryUniversity Hospital InselspitalBernSwitzerland
  4. 4.Vascular Biology CenterMedical College of GeorgiaAugustaUSA

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