, Volume 184, Issue 1, pp 15–19

Matrix Metalloproteinase-1 Activation via Plasmin Generated on Alveolar Epithelial Cell Surfaces

  • Takashi Ishida
  • Hiroki Tsukada
  • Takashi Hasegawa
  • Hirohisa Yoshizawa
  • Fumitake Gejyo


Plasmin is a potent protease related to tissue repair/remodeling not by fibrinolysis alone but also by activation of cytokines such as transforming growth factor and hepatocyte growth factor and by activation of matrix metalloproteases. We examined whether matrix matalloproteinase-1 was activated via plasminogen activation on surfaces of cultured alveolar epithelial cells (A-549). Cells were cultured overnight with plasminogen, pro-matrix metalloproteinase-1, and type I collagen as a substrate. Sodium dodecil sulfate–polyacrylamide gel electrophoresis was used to detect type I collagen degradation in culture supernatant. Collagen degradation corresponded to cell surface plasmin generation. No such finding was seen in the absence of cells or plasminogen. Alveolar epithelial plasminogen activation is important in matrix metalloproteinase-1 activation and thus presumably in tissue remodeling in pulmonary fibrosing pulmonary diseases such as idiopathic pulmonary fibrosis.


Plasminogen Plasmin Alveolar epithelial cells Matrix metalloproteinase Tissue remodeling 


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Takashi Ishida
    • 1
  • Hiroki Tsukada
    • 1
  • Takashi Hasegawa
    • 1
  • Hirohisa Yoshizawa
    • 1
  • Fumitake Gejyo
    • 1
  1. 1.Division of Respiratory MedicineNiigata University Graduate School of Medical and Dental SciencesNiigataJapan

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