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European Archives of Oto-Rhino-Laryngology

, Volume 257, Issue 4, pp 199–204 | Cite as

Effects of macrolides on interleukin-8 secretion from human nasal epithelial cells

  • K. Fujita
  • Takeshi Shimizu
  • Yuich Majima
  • Yasuo Sakakura
Rhinology

Abstract

Low-dose, long-term macrolide treatment has recently been reported to be very effective in patients with chronic airway diseases. We examined the in vivo and in vitro effects of 14-membered macrolide antibiotics erythromycin (EM) and clarithromycin (CAM) on interleukin (IL)-8 secretion from human nasal epithelial cells. Fifteen patients with chronic sinusitis received macrolide treatment (CAM 400 mg/day) for 1 to 3 months. The number of infiltrated neutrophils and IL-8 concentrations in the nasal discharges of these patients decreased significantly at 1 to 2 months after the treatment. In vitro effects of EM and CAM on IL-8 secretion were examined in nasal epithelial cells cultured at the air-liquid interface. After 14-day culture in the air-liquid interface, macrolide antibiotics were added in medium for 24 h. EM and CAM at concentrations of 10–4 M did not affect spontaneous secretions or IL-1β-induced secretions of IL-8 either apically or basolaterally. When cells were preincubated with 10–4 M CAM for 7 days, the IL-1β-induced secretion of IL-8 decreased significantly. However, no difference was observed between the effects of 10–4 M CAM and 10–4 M josamycin, a 16-membered macrolide. These results suggest that macrolide treatment inhibits neutrophil infiltration and IL-8 secretion in nasal epithelium in vivo and that these clinical effects depend on a mechanism other than the direct action of macrolide on nasal epithelial cells.

Key words Chronic sinusitis Interleukin-8 Human nasal epithelial cells Macrolide antibiotics 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • K. Fujita
    • 1
  • Takeshi Shimizu
    • 1
  • Yuich Majima
    • 1
  • Yasuo Sakakura
    • 1
  1. 1.Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, Japan Tel.: ++81-59-232-1111, ext. 5637, Fax: ++81-59-231-5218JP

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