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European Archives of Oto-Rhino-Laryngology

, Volume 255, Issue 3, pp 127–131 | Cite as

Apoptosis of guinea pig cochlear hair cells following chronic aminoglycoside treatment

  • T. Nakagawa
  • H. Yamane
  • M. Takayama
  • K. Sunami
  • Y. Nakai
Otology

Abstract

Although aminoglycosides have been investigated for their cochleotoxicity, it has still not been determined whether apoptosis or necrosis results in cochlear hair cell death following aminoglycoside treatment. To study possible mechanisms of cell death, we used in situ DNA break-labeling to examine guinea pig cochleae affected by kanamycin ototoxicity. Chronic kanamycin treatment induced DNA fragmentation that was detectable in both outer and inner hair cells, suggesting the occurrence of apoptosis. These findings suggest that apoptosis achieves deletion of affected hair cells without disrupting tissue architecture in the organ of Corti.

Key words Cochlea Aminoglycoside toxicity Hair cell apoptosis Nick-end labeling 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • T. Nakagawa
    • 1
  • H. Yamane
    • 2
  • M. Takayama
    • 2
  • K. Sunami
    • 2
  • Y. Nakai
    • 2
  1. 1.Department of Otorhinolaryngology, Yodogawa Christian Hospital, 2-9-26 Awaji Higashiyodogawa-ku, Osaka 533, JapanJP
  2. 2.Department of Otorhinolaryngology, Osaka City University School of Medicine, Osaka, JapanJP

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