European Archives of Oto-Rhino-Laryngology

, Volume 275, Issue 9, pp 2253–2257 | Cite as

TNF-alpha G/A308 polymorphism association with nasal polyposis in North part of Iran

  • Masoumeh Faghani
  • Parvaneh Keshavars
  • Alireza Sharafshah
  • Babak Pourgholamali
  • Farshad Moharami
  • Shadman NematiEmail author


Nasal Polyposis (NP) is a complex multi-factorial disease; associated with several environmental, genetic and inflammatory factors. TNF-alpha is one of the major pro-inflammatory cytokines involved in NP pathogenesis. Some of the polymorphisms of this gene affect its expression. Therefore, the aim of this study was to evaluate the polymorphism of TNF-alpha G/A308 gene and its association with nasal polyposis in north part of Iran. In this case-control study, 60 patients with NP and 75 healthy individuals referred to Guilan University hospital were evaluated. After DNA extraction, RFLP-PCR was used to determine polymorphism. Chi-square test was used to compare the frequency distribution of genotype and alleles of TNF-alpha gene with NP. The frequency of genotype G/G, A/A and G/A in the NP group was 46.7, 10.0 and 43.3%, and in the control group was 65.3, 5.3 and 29.3%, respectively. There was a statistically significant difference between genotype G/G in two groups (P = 0.006, OR 2.58, CI 1.27–5.23). In addition, the frequency of allele A in patients and controls was 31.7 and 20%, respectively; and this difference was statistically significant (p = 0.02). The findings of this study demonstrated that polymorphism in TNF-alpha gene might be a risk factor for NP in north part of Iran and the minor frequency of G308A allele in the current study is slightly more than other major populations. However, more investigations with high number of population are necessary in future.


Nasal polyps Tumor necrosis factor-alpha Polymorphism/single nucleotide Iran Polymerase chain reaction 



The authors would like to acknowledge vice chancellor of research, Guilan University of Medical Sciences for supporting this research.


This work is supported by the Guilan University of Medical Sciences by Grant number: 93090408, approved by University ethic committee (ethic code: 1930400407).

Compliance with ethical standards

Conflict of interest

The authors declare that there was no any conflict of Interest during performing this study.


  1. 1.
    Pasaje CF, Bae JS, Park BL, Cheong HS, Kim JH, Jang AS, Uh ST, Park CS, Shin HD (2012) Possible role of EMID2 on nasal polyps pathogenesis in Korean asthma patients. BMC Med Genet 13:2. CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Bernstein JM (2001) The molecular biology of nasal polyposis. Curr Allergy Asthma Rep 1(3):262–267CrossRefPubMedGoogle Scholar
  3. 3.
    Berghea EC, Popa OM, Meirosu M, Popa LO, Bara C, Bumbacea RS (2014) Association of TNF-alpha gene polymorphism with nasal polyposis in Romanian asthmatic patients. Rom J Rhinol 4(15):149–155Google Scholar
  4. 4.
    Batikhan H, Gokcan MK, Beder E, Akar N, Ozturk A, Gerceker M (2010) Association of the tumor necrosis factor-alpha—308 G/A polymorphism with nasal polyposis. Eur Arch Otorhinolaryngol 267(6):903–908. CrossRefPubMedGoogle Scholar
  5. 5.
    Erbek SS, Yurtcu E, Erbek S, Atac FB, Sahin FI, Cakmak O (2007) Proinflammatory cytokine single nucleotide polymorphisms in nasal polyposis. Arch Otolaryngol Head Neck Surg 133(7):705–709. CrossRefPubMedGoogle Scholar
  6. 6.
    Staikuniene J, Vaitkus S, Japertiene LM, Ryskiene S (2008) Association of chronic rhinosinusitis with nasal polyps and asthma: clinical and radiological features, allergy and inflammation markers. Medicina 44(4):257–265CrossRefPubMedGoogle Scholar
  7. 7.
    Bernstein JM, Anon JB, Rontal M, Conroy J, Wang C, Sucheston L (2009) Genetic polymorphisms in chronic hyperplastic sinusitis with nasal polyposis. The Laryngoscope 119(7):1258–1264. CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Saji F, Nonaka M, Pawankar R (2000) Expression of RANTES by IL-1 beta and TNF-alpha stimulated nasal polyp fibroblasts. Auris Nasus Larynx 27(3):247–252CrossRefPubMedGoogle Scholar
  9. 9.
    Mekinian A, Tamouza R, Pavy S, Gestermann N, Ittah M, Mariette X, Miceli-Richard C (2011) Functional study of TNF-alpha promoter polymorphisms: literature review and meta-analysis. Eur Cytokine Netw 22(2):88–102. PubMedCrossRefGoogle Scholar
  10. 10.
    Rugina M, Serrano E, Klossek J, Crampette L, Stoll D, Bebear J, Perrahia M, Rouvier P, Peynegre R (2002) Epidemiological and clinical aspects of nasal polyposis in France; the ORLI group experience. Rhinology 40(2):75–79PubMedGoogle Scholar
  11. 11.
    Ardebili SM, Yeghaneh T, Gharesouran J, Rezazadeh M, Farhoudi M, Ayromlou H, Talebi M, Ghojazadeh M (2011) Genetic association of TNF-alpha-308 G/A and – 863 C/A polymorphisms with late onset Alzheimer’s disease in Azeri Turk population of Iran. J Res Med Sci 16(8):1006–1013PubMedPubMedCentralGoogle Scholar
  12. 12.
    Hajeer AH, Hutchinson IV (2001) Influence of TNFα gene polymorphisms on TNFα production and disease. Hum Immunol 62(11):1191–1199CrossRefPubMedGoogle Scholar
  13. 13.
    Meymane Jahromi A, Shahabi Pour A (2012) The epidemiological and clinical aspects of nasal polyps that require surgery. Iran J Otorhinolaryngol 24(67):75–78PubMedPubMedCentralGoogle Scholar
  14. 14.
    Gerami H, Koosha A, Parast F, Karimi H (2007) Survey the frequency of associated factors with polyps in patients who polypectomized at Amir-Al-Momenin Hospital/Rasht. J Guilan Univ Med Sci 16(62):14–19Google Scholar
  15. 15.
    Hashemian F, Farahani F (2005) Frequency of Nasal Polyposis in Chronic Rhinosinusitis and Role of Endoscopic Examination in Correct Diagnosis. Sci J Hamadan Univ Med Sci 12(3):20–23Google Scholar
  16. 16.
    Larsen K, Tos M (2002) The estimated incidence of symptomatic nasal polyps. Acta Otolaryngol 122(2):179–182CrossRefPubMedGoogle Scholar
  17. 17.
    Nemati S, Mojtahedi A, Naghavi SE et al (2012) Investigating Helicobacter pylori in nasal polyposis using polymerase chain reaction, urease test and culture. Eur Arch Otorhinolaryngol 269:1457–1461. CrossRefPubMedGoogle Scholar
  18. 18.
    Krunkosky TM, Fischer BM, Martin LD, Jones N, Akley NJ, Adler KB (2000) Effects of TNF-alpha on expression of ICAM-1 in human airway epithelial cells in vitro. Signaling pathways controlling surface and gene expression. Am J Respir Cell Mol Biol 22(6):685–692. CrossRefPubMedGoogle Scholar
  19. 19.
    Baz K, Emin Erdal M, Yazici AC, Soylemez F, Guvenc U, Tasdelen B, Ikizoglu G (2008) Association between tumor necrosis factor-alpha gene promoter polymorphism at position—308 and acne in Turkish patients. Arch Dermatol Res 300(7):371–376. CrossRefPubMedGoogle Scholar
  20. 20.
    İsmi O, Ozcan C, Polat G, Kul S, Gorur K, Puturgeli T (2017) TNF-[alpha] and IL-1 [beta] cytokine gene polymorphism in patients with nasal polyposis. Turk Arch Otorhinolaryngol Turk Otorinolarengoloji Arsivi 55(2):51–56CrossRefPubMedGoogle Scholar
  21. 21.
    Golshani H, Haghani K, Dousti M, Bakhtiyari S (2015) Association of TNF-alpha 308 G/A polymorphism with type 2 diabetes: A case-control study in the Iranian Kurdish Ethnic Group. Osong Public Health Res Perspect 6(2):94–99. CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Collins MM, Pang YT, Loughran S, Wilson JA (2002) Environmental risk factors and gender in nasal polyposis. Clin Otolaryngol Allied Sci 27(5):314–317CrossRefPubMedGoogle Scholar
  23. 23.
    Busaba NY, Sin HJ, Salman SD (2008) Impact of gender on clinical presentation of chronic rhinosinusitis with and without polyposis. J Laryngol Otol 122(11):1180–1184. CrossRefPubMedGoogle Scholar
  24. 24.
    Finotto S, Ohno I, Marshall JS, Gauldie J, Denburg JA, Dolovich J, Clark DA, Jordana M (1994) TNF-alpha production by eosinophils in upper airways inflammation (nasal polyposis). J Immunol 153(5):2278–2289PubMedGoogle Scholar
  25. 25.
    Quasney MW, Bronstein DE, Cantor RM, Zhang Q, Stroupe C, Shike H, Bastian JF, Matsubara T, Fujiwara M, Akimoto K, Newburger JW, Burns JC (2001) Increased frequency of alleles associated with elevated tumor necrosis factor-alpha levels in children with Kawasaki disease. Pediatr Res 49(5):686–690. CrossRefPubMedGoogle Scholar
  26. 26.
    Szabo K, Kiricsi A, Revesz M, Vona I, Szabo Z, Bella Z, Polyanka H, Kadocsa E, Kemeny L, Szell M, Hirschberg A (2013) The – 308 G> A SNP of TNFA is a factor predisposing to chronic rhinosinusitis associated with nasal polyposis in aspirin-sensitive Hungarian individuals: conclusions of a genetic study with multiple stratifications. Int Immunol 25(6):383–388. CrossRefPubMedGoogle Scholar
  27. 27.
    Pearlman A, Chandra R, Conley D (2010) Epidemiology of nasal polyps. Nasal polyposis. Pathogenesis, medical and surgical treatment. (Eds) Önerci TM, Ferguson BJ. Springer, BerlinGoogle Scholar
  28. 28.
    Klossek JM, Neukirch F, Pribil C, Jankowski R, Serrano E, Chanal I, El Hasnaoui A (2005) Prevalence of nasal polyposis in France: a cross-sectional, case-control study. Allergy 60(2):233–237. CrossRefPubMedGoogle Scholar
  29. 29.
    Larsen K (1996) The clinical relationship of nasal polyps to asthma. Allergy Asthma Proc 17 (5):243–249CrossRefGoogle Scholar
  30. 30.
    Sharifi K, Rostami F, Faam B, Daneshpour MS, Azizi F, Hedayati M (2011) Association of TNF-α promoter G-308A polymorphism and body mass index and other obesity effective factors in Tehran lipid and glucose study. Razi J Med Sci 18(86):39–48Google Scholar
  31. 31.
    Nourian M, Asgharian AM, Asadzadeh Aghdae H (2016) Lack of association Between tumor necrosis factor alpha (TNFα) Gene—1031C/T polymorphisms and susceptibility to inflammatory bowel disease (IBD). Arak Med Univ J (AMUJ) 19(108):71–79Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Anatomical Sciences Department, Faculty of MedicineGuilan University of Medical SciencesRashtIran
  2. 2.Cellular and Molecular Research Center, Faculty of MedicineGuilan University of Medical SciencesRashtIran
  3. 3.Rino-sinus, Ear, and Skull base Diseases Research Center, Amir-al Momenin University HospitalGuilan University of Medical SciencesRashtIran

Personalised recommendations