European Archives of Oto-Rhino-Laryngology

, Volume 274, Issue 1, pp 215–222 | Cite as

Differential protein expression in the secretory fluids of maxillary sinusitis and maxillary retention cyst

  • Soung Min Kim
  • Mi Young Eo
  • Yun Ju Cho
  • Yeon Sook Kim
  • Suk Keun LeeEmail author


Both maxillary sinusitis (MS) and maxillary retention cyst (MRC) involve the maxillary sinus and show similar clinical features. Clinically, differentiating between MS and MRC is sometimes difficult in asymptomatic patients, despite their quite different pathogenic behaviors. To identify differential protein expressions in the secretory fluids of MS and MRC, 25 cases of asymptomatic MS and 15 cases of asymptomatic MRC were examined pathologically in this study. All patients underwent routine endoscopic sinus surgery or modified Caldwell-Luc procedure and the sinus mucosal specimens obtained during these procedures with the approval of the Institutional Review Board. Their secretory fluids were analyzed via immunoprecipitation-based high-performance liquid chromatography (IP-HPLC) using 25 types of antiserum, including inflammatory cytokines, antimicrobial proteins, and mucosal protective proteins. In the histological examinations, MS and MRC showed similar features in the secretory columnar epithelial lining and thick submucosal connective tissue, both of which contained few inflammatory cells infiltrates. The IP-HPLC analysis revealed that TNFα, IL-1, -8, MMP-3, -10, α1-antitrypsin, cathepsin C, lysozyme, lactoferrin, β-defensin-1, -3, LL-37, mucocidin, and mucin-1 were more intensely expressed in MS than in MRC; whereas IgA, cystatin A, and proline-rich proteins were more strongly expressed in MRC than in MS. These data indicate that the secretory fluid of MS is indicative of a more robust inflammatory reaction to certain bacteria compared to that of MRC, while the secretory fluid of MRC contains more abundant mucosal protective proteins compared to that of MS. Taken together, the IP-HPLC analysis of MS and MRC secretory fluid revealed that MRC showed a weaker inflammatory reaction but a stronger mucosal protective function than MS.


Maxillary sinusitis (MS) Maxillary retention cyst (MRC) Protein expression IP-HPLC 



This study was supported by a Grant of the Korean Health Technology R&D Project, Ministry of Health and Welfare, and Republic of Korea (HI15C0689).

Compliance with ethical standards

Conflicts of interest

There are no conflicts of interest in this article.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional Review Board (IRB2015-07) at Gangneung National University Hospital and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Soung Min Kim
    • 1
  • Mi Young Eo
    • 1
  • Yun Ju Cho
    • 1
  • Yeon Sook Kim
    • 2
  • Suk Keun Lee
    • 3
    Email author
  1. 1.Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Research InstituteSeoul National UniversitySeoulKorea
  2. 2.Department of Dental HygieneCheongju UniversityCheongjuKorea
  3. 3.Department of Oral Pathology, College of DentistryGangneung-Wonju National UniversityGangneungKorea

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