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European Archives of Oto-Rhino-Laryngology

, Volume 274, Issue 1, pp 215–222 | Cite as

Differential protein expression in the secretory fluids of maxillary sinusitis and maxillary retention cyst

  • Soung Min Kim
  • Mi Young Eo
  • Yun Ju Cho
  • Yeon Sook Kim
  • Suk Keun LeeEmail author
Rhinology

Abstract

Both maxillary sinusitis (MS) and maxillary retention cyst (MRC) involve the maxillary sinus and show similar clinical features. Clinically, differentiating between MS and MRC is sometimes difficult in asymptomatic patients, despite their quite different pathogenic behaviors. To identify differential protein expressions in the secretory fluids of MS and MRC, 25 cases of asymptomatic MS and 15 cases of asymptomatic MRC were examined pathologically in this study. All patients underwent routine endoscopic sinus surgery or modified Caldwell-Luc procedure and the sinus mucosal specimens obtained during these procedures with the approval of the Institutional Review Board. Their secretory fluids were analyzed via immunoprecipitation-based high-performance liquid chromatography (IP-HPLC) using 25 types of antiserum, including inflammatory cytokines, antimicrobial proteins, and mucosal protective proteins. In the histological examinations, MS and MRC showed similar features in the secretory columnar epithelial lining and thick submucosal connective tissue, both of which contained few inflammatory cells infiltrates. The IP-HPLC analysis revealed that TNFα, IL-1, -8, MMP-3, -10, α1-antitrypsin, cathepsin C, lysozyme, lactoferrin, β-defensin-1, -3, LL-37, mucocidin, and mucin-1 were more intensely expressed in MS than in MRC; whereas IgA, cystatin A, and proline-rich proteins were more strongly expressed in MRC than in MS. These data indicate that the secretory fluid of MS is indicative of a more robust inflammatory reaction to certain bacteria compared to that of MRC, while the secretory fluid of MRC contains more abundant mucosal protective proteins compared to that of MS. Taken together, the IP-HPLC analysis of MS and MRC secretory fluid revealed that MRC showed a weaker inflammatory reaction but a stronger mucosal protective function than MS.

Keywords

Maxillary sinusitis (MS) Maxillary retention cyst (MRC) Protein expression IP-HPLC 

Notes

Acknowledgments

This study was supported by a Grant of the Korean Health Technology R&D Project, Ministry of Health and Welfare, and Republic of Korea (HI15C0689).

Compliance with ethical standards

Conflicts of interest

There are no conflicts of interest in this article.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional Review Board (IRB2015-07) at Gangneung National University Hospital and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

References

  1. 1.
    Park YW, Kim JH, Kim YH et al (2008) Antral polyp versus maxillary sinusitis. J Korean Assoc Maxillofac Plast Reconstr Surg 30:489–494Google Scholar
  2. 2.
    Kim YS, An KY, Lee SK (2010) Pathological findings for mucous retention cyst in maxillary sinus. Korean J Oral Maxillofac Pathol 34:9–18Google Scholar
  3. 3.
    Donizeth-Rodrigues C, Fonseca-Da Silveira M, Goncalves-De Alencar AH et al (2013) Three-dimensional images contribute to the diagnosis of mucous retention cyst in maxillary sinus. Med Oral Patol Oral Cir Bucal 18:e151–e157CrossRefPubMedGoogle Scholar
  4. 4.
    Benevides GN, Salgado GA Jr, Ferreira CR et al (2015) Bacterial sinusitis and its frightening complications: subdural empyema and Lemierre syndrome. Autopsy Case Rep 5:19–26CrossRefGoogle Scholar
  5. 5.
    Banglawala SM, Schlosser RJ, Wenztel J et al (2016) Trends in chronic rhinosinusitis research in the past three decades. Int Forum Allergy Rhinol 6:46–51CrossRefPubMedGoogle Scholar
  6. 6.
    Gardner DG (1984) Pseudocysts and retention cysts of the maxillary sinus. Oral Surg Oral Med Oral Pathol 58:561–567CrossRefPubMedGoogle Scholar
  7. 7.
    Habesoglu TE, Habesoglu M, Surmeli M et al (2010) Unilateral sinonasal symptoms. J Craniofac Surg 21:2019–2022CrossRefPubMedGoogle Scholar
  8. 8.
    Marcal Vieira EM, de Morais S, de Musis CR et al (2015) Frequency of maxillary sinus mucous retention cysts in a central Brazilian population. J Dent (Shiraz) 16:169–174Google Scholar
  9. 9.
    Rodrigues CD, Freire GF, Silva LB et al (2009) Prevalence and risk factors of mucous retention cysts in a Brazilian population. Dentomaxillofac Radiol 38:480–483CrossRefPubMedGoogle Scholar
  10. 10.
    Lee JT, Escobar OH, Anouseyan R et al (2014) Assessment of epithelial innate antimicrobial factors in sinus tissue from patients with and without chronic rhinosinusitis. Int Forum Allergy Rhinol 4:893–900CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Zustin J, Scheuer HA, Knecht R (2013) Enhanced podoplanin expression in chronic maxillary sinusitis. In Vivo 27:551–554PubMedGoogle Scholar
  12. 12.
    Kim YS (2015) Protein expression changes induced by cisplatin in an oral cancer cell line as determined by immunoprecipitation-based high performance liquid chromatography. Korean J Oral Maxillofac Pathol 39:567–582CrossRefGoogle Scholar
  13. 13.
    Kim YS, Lee SK (2015) IP-HPLC analysis of human salivary protein complexes. Korean J Oral Maxillofac Pathol 39:615–622CrossRefGoogle Scholar
  14. 14.
    Kim YS, Lee SK, Park SC et al (2005) Cloning and identification of a new antimicrobial protein, salvic, from human salivary gland. In: International symposium of Maxillofacial and Oral Regenerative Biology in Okayama, vol 1, pp 258–260Google Scholar
  15. 15.
    Kim YS, Lim JG, Lee SS et al (2006) Polyclonal multiple antibodies against proteins cross-linked on oral exfoliated squamous cells. Korean J Gerontol 16:164–170Google Scholar
  16. 16.
    Kim YS, Lee SK (2009) Aggregation of salivary mucin-1 and proline rich proteins by transglutaminase 4 in a huge sialolith with central nidus of bacterial colony. Korean J Oral Maxillofac Pathol 33:239–244Google Scholar
  17. 17.
    Daele J, Zicot AF (2000) Humoral immunodeficiency in recurrent upper respiratory tract infections: some basic, clinical and therapeutic features. Acta Otorhinolaryngol Belg 54:373–390PubMedGoogle Scholar
  18. 18.
    Xu JH, Dai WJ, Chen B et al (2015) Mucosal immunization with PsaA protein, using chitosan as a delivery system, increases protection against acute otitis media and invasive infection by Streptococcus pneumoniae. Scand J Immunol 81:177–185CrossRefPubMedGoogle Scholar
  19. 19.
    Carothers DG, Graham SM, Jia HP et al (2001) Production of beta-defensin antimicrobial peptides by maxillary sinus mucosa. Am J Rhinol 15:175–179CrossRefPubMedGoogle Scholar
  20. 20.
    Kaliner MA (1992) Human nasal host defense and sinusitis. J Allergy Clin Immunol 90(3 Pt 2):424–430CrossRefPubMedGoogle Scholar
  21. 21.
    Stoeckelhuber M, Olzowy B, Ihler F et al (2014) Immunolocalization of antimicrobial and cytoskeletal components in the serous glands of human sinonasal mucosa. Histol Histopathol 29:1315–1324PubMedGoogle Scholar
  22. 22.
    Woods CM, Lee VS, Hussey DJ et al (2012) Lysozyme expression is increased in the sinus mucosa of patients with chronic rhinosinusitis. Rhinology 50:147–156PubMedGoogle Scholar
  23. 23.
    Jyonouchi H, Sun S, Kennedy CA et al (2000) Interferon gamma levels in the sinus, ear, and airway in a rabbit sinusitis model induced by Bacteroides inoculation. Arch Otolaryngol Head Neck Surg 126:529–532CrossRefPubMedGoogle Scholar
  24. 24.
    Elmorsy S, El-Naggar MM, Abdel-Aal SM et al (2010) Sinus aspirates in chronic rhinosinusitis: fungal colonization of paranasal sinuses, evaluation of ICAM-1 and IL-8 and studying of immunological effect of long-term macrolide therapy. Rhinology 48:312–317PubMedGoogle Scholar
  25. 25.
    Tokushige E, Itoh K, Ushikai M et al (1994) Localization of IL-1 beta mRNA and cell adhesion molecules in the maxillary sinus mucosa of patients with chronic sinusitis. Laryngoscope 104:1245–1250CrossRefPubMedGoogle Scholar
  26. 26.
    Detwiller KY, Smith TL, Mace JC et al (2013) Steroid-independent upregulation of matrix metalloproteinase 9 in chronic rhinosinusitis patients with radiographic evidence of osteitis. Int Forum Allergy Rhinol 3:364–368CrossRefPubMedPubMedCentralGoogle Scholar
  27. 27.
    Hamaguchi Y, Ohi M, Sakakura Y et al (1986) Significance of lysosomal proteases; cathepsins B and H in maxillary mucosa and nasal polyp with non-atopic chronic inflammation. Rhinology 24:187–194PubMedGoogle Scholar
  28. 28.
    Kay NJ, Terry RM, Swinburne L (1986) Protease inhibitors in maxillary antral mucosa. J Laryngol Otol 100:289–290CrossRefPubMedGoogle Scholar
  29. 29.
    Lee CW, Kim TH, Lee HM et al (2009) Upregulation of elafin and cystatin C in the ethmoid sinus mucosa of patients with chronic sinusitis. Arch Otolaryngol Head Neck Surg 135:771–775CrossRefPubMedGoogle Scholar
  30. 30.
    Woods CM, Hooper DN, Ooi EH et al (2012) Fungicidal activity of lysozyme is inhibited in vitro by commercial sinus irrigation solutions. Am J Rhinol Allergy 26:298–301CrossRefPubMedGoogle Scholar
  31. 31.
    Woods CM, Hooper DN, Ooi EH et al (2011) Human lysozyme has fungicidal activity against nasal fungi. Am J Rhinol Allergy 25:236–240CrossRefPubMedGoogle Scholar
  32. 32.
    Seshadri S, Lin DC, Rosati M et al (2012) Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis. Allergy 67:920–928CrossRefPubMedPubMedCentralGoogle Scholar
  33. 33.
    Claeys S, de Belder T, Holtappels G et al (2003) Human beta-defensins and toll-like receptors in the upper airway. Allergy 58:748–753CrossRefPubMedGoogle Scholar
  34. 34.
    Chennupati SK, Chiu AG, Tamashiro E et al (2009) Effects of an LL-37-derived antimicrobial peptide in an animal model of biofilm Pseudomonas sinusitis. Am J Rhinol Allergy 23:46–51CrossRefPubMedGoogle Scholar
  35. 35.
    Takeuchi K, Yuta A, Sakakura Y (1995) MUC2 mucin gene expression in the nose and maxillary sinus. Am J Otolaryngol 16:391–395CrossRefPubMedGoogle Scholar
  36. 36.
    Sakurada K, Akutsu T, Watanabe K et al (2012) Identification of nasal blood by real-time RT-PCR. Leg Med (Tokyo) 14:201–204CrossRefGoogle Scholar
  37. 37.
    Carenfelt C (1977) Maxillary sinusitis. Effects of treatment on the local antibacterial defence. Acta Otolaryngol 84:440–445CrossRefPubMedGoogle Scholar
  38. 38.
    Tesfaigzi J, Th’ng J, Hotchkiss JA et al (1996) A small proline-rich protein, SPRR1, is upregulated early during tobacco smoke-induced squamous metaplasia in rat nasal epithelia. Am J Respir Cell Mol Biol 14:478–486CrossRefPubMedGoogle Scholar
  39. 39.
    Warner TF, Azen EA (1984) Proline-rich proteins are present in serous cells of submucosal glands in the respiratory tract. Am Rev Respir Dis 130:115–118PubMedGoogle Scholar
  40. 40.
    Takabayashi T, Kato A, Peters AT et al (2013) Increased expression of factor XIII-A in patients with chronic rhinosinusitis with nasal polyps. J Allergy ClinImmunol 132:584–592CrossRefGoogle Scholar
  41. 41.
    Albu S (2010) Symptomatic maxillary sinus retention cysts: should they be removed? Laryngoscope 120:1904–1909CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Soung Min Kim
    • 1
  • Mi Young Eo
    • 1
  • Yun Ju Cho
    • 1
  • Yeon Sook Kim
    • 2
  • Suk Keun Lee
    • 3
    Email author
  1. 1.Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Research InstituteSeoul National UniversitySeoulKorea
  2. 2.Department of Dental HygieneCheongju UniversityCheongjuKorea
  3. 3.Department of Oral Pathology, College of DentistryGangneung-Wonju National UniversityGangneungKorea

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