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European Archives of Oto-Rhino-Laryngology

, Volume 273, Issue 12, pp 4127–4133 | Cite as

Histological identification of nasopharyngeal mechanoreceptors

  • Florent Salburgo
  • Stéphane Garcia
  • Aude Lagier
  • Dominique Estève
  • Jean-Pierre Lavieille
  • Marion Montava
Otology

Abstract

The auditory tube plays a fundamental role in regulating middle ear pressure. A “system” sensitive to a pressure gradient between the middle ear and the ambient environment is necessary. The presence of mechanoreceptors in the middle ear and the tympanic membrane has been studied, but the presence of these receptors in the nasopharyngeal region remains unclear. The aim of this study is to confirm the presence of pressure sensitive corpuscles in the nasopharynx. An experimental study was conducted on five fresh and unembalded human cadavers. The pharyngeal ostium of the auditory tube and its periphery was removed in one piece by video-assisted endonasal endoscopy. Samples were fixed in formaldehyde solution, embedded in paraffin, and cut. Slides were analyzed by HES (Hematoxyline Eosine Safran) coloration, by S100 protein and neurofilament protein immunostaining. Encapsulated nerve endings were researched and identified by slides analysis. Eight samples were included in our study. On seven samples, Ruffini corpuscles were identified in the mucosa of the posterior area of the pharyngeal ostium, with a higher concentration in the pharyngeal recess and in the posterior nasopharyngeal wall. Our study identified nasopharyngeal mechanoreceptors that could detect the nasopharyngeal pressure and, by extension, the atmospheric pressure. These findings support the theory of the neuronal reflex arc of isobaric system of the middle ear, based on the existence of a “system” sensitive to a pressure gradient between the middle ear and the ambient environment. Understanding of this system has been helpful in the diagnosis and management of middle ear diseases.

Keywords

Auditory tube Eustachian tube Sensory receptors Mechanoreceptors Nasopharynx Middle ear pressure 

Notes

Acknowledgment

No financial support received.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest concerning this article.

Research involving human participants and/or animals

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.

References

  1. 1.
    Bluestone CD (1983) Eustachian tube function: physiology, pathophysiology, and role of allergy in pathogenesis of otitis media. J Allergy Clin Immunol 72:242–251CrossRefPubMedGoogle Scholar
  2. 2.
    Elner A (1976) Normal gas exchange in the human middle ear. Ann Otol Rhinol Laryngol 85:161–164PubMedGoogle Scholar
  3. 3.
    Sadé J, Luntz M, Yaniv E, Yurovitzki E, Berger G, Galrenter I (1986) The eustachian tube lumen in chronic otitis media. Am J Otol 7:439–442PubMedGoogle Scholar
  4. 4.
    Freeman MA, Wyke B (1966) Articular contributions to limb muscle reflexes. The effects of partial neurectomy of the knee-joint on postural reflexes. Br J Surg 53:61–68CrossRefPubMedGoogle Scholar
  5. 5.
    Gussen R (1970) Pacinian corpuscles in the middle ear. J Laryngol Otol 84:71–76CrossRefPubMedGoogle Scholar
  6. 6.
    Lim DJ (1970) Human tympanic membrane. An ultrastructural observation. Acta Otolaryngol 70:176–186CrossRefPubMedGoogle Scholar
  7. 7.
    Nagai T, Tono T (1989) Encapsulated nerve corpuscles in the human tympanic membrane. Arch Otorhinolaryngol 246:169–172CrossRefPubMedGoogle Scholar
  8. 8.
    Nagai T, Nagai M, Nagata Y, Morimitsu T (1989) The effects of anesthesia of the tympanic membrane on eustachian tube function. Arch Otorhinolaryngol 246:210–212CrossRefPubMedGoogle Scholar
  9. 9.
    Esteve D, Dubreuil C, Vella Vedova C, Normand B, Lavieille J-P, Martin C (2001) Physiology and physiopathology of the Eustachian tube opening function: interest of tubomanometry. JF ORL 50:233–241Google Scholar
  10. 10.
    Songu M, Aslan A, Unlu HH, Celik O (2009) Neural control of eustachian tube function. Laryngoscope 119:1198–1202CrossRefPubMedGoogle Scholar
  11. 11.
    Guindi GM (1981) Nasopharyngeal mechanoreceptors and their role in autoregulation of endotympanic pressure. ORL J Otorhinolaryngol Relat Spec 43:56–60CrossRefPubMedGoogle Scholar
  12. 12.
    Eden AR (1981) Neural connections between the middle ear, eustachian tube and brain. Implications for the reflex control of middle ear aeration. Ann Otol Rhinol Laryngol 90:566–569CrossRefPubMedGoogle Scholar
  13. 13.
    Eden AR, Gannon PJ (1987) Neural control of middle ear aeration. Arch Otolaryngol Head Neck Surg 113:133–137CrossRefPubMedGoogle Scholar
  14. 14.
    Eden AR, Laitman JT, Gannon PJ (1990) Mechanisms of middle ear aeration: anatomic and physiologic evidence in primates. Laryngoscope 100:67–75CrossRefPubMedGoogle Scholar
  15. 15.
    Dirckx JJ, Decraemer WF, von Unge M, Larsson C (1998) Volume displacement of the gerbil eardrum pars flaccida as a function of middle ear pressure. Hear Res 118:35–46CrossRefPubMedGoogle Scholar
  16. 16.
    Didyk LA, Dirckx JJ, Bogdanov VB, Lysenko VA, Gorgo YP (2007) The mechanical reaction of the pars flaccida of the eardrum to rapid air pressure oscillations modeling different levels of atmospheric disturbances. Hear Res 223:20–28CrossRefPubMedGoogle Scholar
  17. 17.
    Kanagasuntheram R, Wong WC, Chan HL (1969) Some observations on the innervation of the human nasopharynx. J Anat 104:361–376PubMedPubMedCentralGoogle Scholar
  18. 18.
    Witherspoon JW, Smirnova IV, McIff TE (2014) Improved gold chloride staining method for anatomical analysis of sensory nerve endings in the shoulder capsule and labrum as examples of loose and dense fibrous tissues. Biotech Histochem 89:355–370CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Lee BI, Min KD, Choi HS, Kwon SW, Chun DI, Yun ES, Lee DW, Jin SY, Yoo JH (2009) Immunohistochemical study of mechanoreceptors in the tibial remnant of the ruptured anterior cruciate ligament in human knees. Knee Surg Sports Traumatol Arthrosc 17:1095–1101CrossRefPubMedGoogle Scholar
  20. 20.
    Sonnery-Cottet B, Bazille C, Hulet C, Colombet P, Cucurulo T, Panisset JC, Potel JF, Servien E, Trojani C, Djian P, Graveleau N, Pujol N (2014) Histological features of the ACL remnant in partial tears. Knee 21:1009–1013CrossRefPubMedGoogle Scholar
  21. 21.
    Zimny ML (1988) Mechanoreceptors in articular tissues. Am J Anat 182:16–32CrossRefPubMedGoogle Scholar
  22. 22.
    Gaihede M, Dirckx JJ, Jacobsen H, Aernouts J, Søvsø M, Tveterås K (2010) Middle ear pressure regulation—complementary active actions of the mastoid and the Eustachian tube. Otol Neurotol 31:603–611PubMedGoogle Scholar
  23. 23.
    Collin M, Coulange M, Devèze A, Montava M, Estève D, Lavieille JP (2012) Middle ear barotraumas due to rhinopharyngeal scar tissue: tubomanometry diagnostic and therapeutic contribution. Rev Laryngol Otol Rhinol (Bord) 133:157–161Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Florent Salburgo
    • 1
  • Stéphane Garcia
    • 2
    • 3
  • Aude Lagier
    • 1
    • 4
  • Dominique Estève
    • 1
  • Jean-Pierre Lavieille
    • 1
    • 5
  • Marion Montava
    • 1
    • 5
  1. 1.APHM, Hôpital de la Conception, Service d’oto-rhino-laryngologie et de chirurgie cervico-facialeMarseilleFrance
  2. 2.Aix Marseille Université, INSERM, U1070MarseilleFrance
  3. 3.APHM, Hôpital Nord, Laboratoire d’anatomie pathologiqueMarseilleFrance
  4. 4.Aix Marseille Université, CNRS, LPL, UMR 7309MarseilleFrance
  5. 5.Aix Marseille Université, IFSSTAR, LBA, UMR-T 24MarseilleFrance

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